大鼠保留半肝动脉血供的入肝血流阻断法模型的建立

摘要

Objective To establish the model of rat hepatic blood inflow occlusion without hemihepatic artery control. Methods Animal models were established by three surgical pathways. Pringle maneuver (PR), hemihepatic vascular occlusion (HH) and hepatic blood inflow occlusion without hemihepatic artery control (WH). The operation success rate, rat mortality, rat survival rate, liver pathological changes with 45 minutes hepatic ischemia followed by 24 hour reperfusion were observed. Results Thirty-three Wistar rats were operated and in each group 8 rats had been operated successfully. Operation success rate were respectively 88.9％ (8/9) in group PR, 57.1％ (8/14) in group HH, 80.8％ (8/10) in group WH,the rat survival rate in group PR was only 37.5％ (3/8) (hepatocyte spotty necrosis, ballon degeneration and acidophilic necrosis were observed), it was 100％ (8/8) in group HH, and 87.5 ％ (7/8) in group WH.Mild liver pathological changes were obser ued in group HH and WH compared to group PR.Conclusion Hepatic blood inflow occlusion without hemihepatic artery control is a more simple and safe surgical pathway to establish rat hepatic bloodinflow occlusion model.%目的 建立保留半肝动脉血供的入肝血流阻断方法大鼠肝缺血再灌注损伤模型,探讨该方法操作的安全性.方法 复制了Pringle法、半肝阻断血流阻断法及保留半肝动脉血供的入肝血流阻断法三种肝血流阻断法动物模型,统计了每组成功完成8只所需动物数量,计算了手术成功率.同时观察了三种肝血流阻断方法缺血45 min,再灌注24 h后动物存活率及肝细胞病理学改变.结果 模型制作完成视为手术成功,共对33只大鼠进行了手术.每组成功完成8只,手术成功率分别为:Pringle法组88.9%(8/9)、半肝血流阻断组57.1%(8/14)、保留半肝动脉血供的入肝血流阻断组80.0%(8/10).缺血45 min再灌注24 h,Pfingle法组生存率仅为37.5%(3/8),镜下可见片状肝细胞坏死、多发气球样变及嗜酸性变.而半肝血流阻断组生存率100%(8/8)、保留半肝动脉血供的入肝血流阻断组87.5%(7/8),两者镜下偶见点状或小灶状肝细胞坏死、气球样变及嗜酸性变,肝细胞受损比Pringle法组明显轻.结论 采用保留半肝动脉血供的入肝血流阻断法制作动物模型,操作简单、手术安全.