目的 评估GnRH激动剂与拮抗剂对他莫昔芬诱导的子宫腺肌病模型小鼠种植窗期内膜容受性的影响.方法 使用他莫昔芬诱导子宫腺肌病模型小鼠,模型形成后分成三组分别给予生理盐水、GnRH激动剂、GnRH拮抗剂;另有两组正常小鼠注射GnRH激动剂和生理盐水作为对照.停止注射后与雄鼠合笼,每组小鼠分为两部分,一部分10只在种植窗期取子宫,检测内膜容受性指标整合素b3和HOXA10的蛋白表达以及转录水平;另一部分5只于交配后13.5 d处死并计算胚胎种植数.结果 子宫腺肌病模型小鼠内膜容受性指标(整合素b3和HOXA10)下降而且没有胚胎种植,注射拮抗剂后,该种情况并没有改善;但经过GnRHa处理后内膜容受性升高,共有23个胚胎种植.但GnRHa未能够改善正常小鼠子宫内膜容受性以及胚胎着床数.结论 激动剂可提高子宫腺肌病模型小鼠种植窗期内膜容受性,不能提高正常小鼠内膜容受性,拮抗剂不能提高子宫腺肌病模型小鼠内膜容受性.%Objective To compare the effect of GnRH analogues on endometrium receptivity of adenomyosis mouse model induced by tamoxifen. Methods The mouse model of adomyosis was made through Oral administration of tamoxifen .when adenomyosis was established, mice were randomly divided into three groups and were intraperitoneally injected with normal saline, triptorelin, cetrorelix respectively; there were another two groups of normal mice injected with triptorelin,cetrorelix. After injection, the mice werre mated with the male ones, Then each group mice was divided into two parts, one part would be collected uterus in implant window, and another would be euthanized to calculate the number of embryo on E13.5 d. Result The endometrium receptivity (HOXA10, integrin b3)of the adenomyosis mouse model was decreased and there werre no embryo implanted , and in cetrorelix group ,there was no significantly change. But in triptorelin group, endometrium receptivity was increased and the number of embryo was 23. However, triptorelin could not improve the endometrium receptivity, of normal mice. Conclusion Triptorelin can improve the endometrium receptivity of adenomyosis mice model but not for normal mice, and cetrorelix can not increase endometrium receptiveity.
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