目的 研究重组小鼠白介素12(rmIL-12)对经辐射损伤后小鼠骨髓造血干细胞的修复作用.方法 选用SPF级BALB/c鼠,直接取骨髓造血干细胞或小鼠本身在辐照前后用rmIL-12处理后,检测造血干细胞的数量和凋亡细胞数,探讨rmIL-12对辐射损伤后骨髓造血干细胞的修复功能.结果 不论是直接辐射细胞或小鼠本身,在辐照前后用rmIL-12处理后,rmIL-12具有提高受损骨髓造血干细胞的数量增加,细胞凋亡率减少.结论 rmIL-12对辐射损伤后的造血干细胞具有明显的促进修复作用,这为临床上治疗造血细胞受损提供实验依据.%Objective The ability of recombinant murine interleukin-12 (rmIL-12) to facilitate recovery of bone marrow hematopoietic stem cells (HSC) from irradiation in mice model were evaluated. Method The rmIL-12 treated SPF BALB/c mice or bone marrow hematopoietic stem cells obtained from the animals before and after irradiation were examined for the viable counts and apoptosis of bone marrow hematopoietic stem cells. Results The rmIL-12 treatment before and after irradiation was effective to increase hematopoietic stem cells of suppressed bone marrow and reduce apoptosis both in obtained hematopoietic stem cells and in mice. Conclusion The rmIL-12 had obvious effect on recovery of hematopoietic stem cells from irradiation induced damage. Moreover, this study provided experimental evidence for IL-12 as clinic therapy to treat diseases associated with hematopoietic cytopenia.
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