谷物中脱氧雪腐镰刀菌烯醇含量测定方法的比较

摘要

The two methods (HPLC and ELISA) for the determination of DON in grains were established and compared.The limit of detection (LOD) of ELISA was 0.043 μg/mL,the average recovery rates of corn and wheat samples were 96.8％ and 97.3％ respectively,with average RSD 8.8％ and 4.4％.The LOD of HPLC was 0.1 μg/mL,the average recovery rates of corn and wheat samples were both 92.7％,with average RSD 6.8％ and 3.1％ respectively.The results showed that the ELISA method had higher recovery and simpler sample preparation.%建立了测定谷物中脱氧雪腐镰刀菌烯醇(DON)含量的酶联免疫吸附测定法(ELISA)和高效液相色谱法(HPLC),利用两种方法对谷物样品进行DON含量测定,并对结果进行了比较.结果表明,ELISA法的检测限为0.043μg/mL,玉米、小麦样品的平均回收率分别为96.8％和97.3％；RSD的平均值分别为8.8％和4.4％；HPLC法的检测限为0.1 μmL,玉米、小麦样品的平均回收率均为92.7％,RSD的平均值分别为6.8％和3.1％.结果显示,ELISA法相对HPLC法快速简便、回收率高.