食管鳞状细胞癌组织中RASSF1A蛋白的表达

摘要

目的：检测RASSF1A蛋自在食管鳞状细胞癌(ESCC)组织中的表达情况。方法：采用免疫组化法检测ESCC组织(n=245)和正常食管黏膜组织(n=201)的组织芯片中RASSF1A蛋白的表达情况，并探讨其与ESCC患者年龄、性别、肿瘤细胞分化程度、淋巴结转移、TNM分期、大体分型和5 a生存率的关系。结果：ESCC组织中RASSF1A蛋白阳性表达率低于正常食管黏膜组织中的阳性表达率(46.9％vs73.1％；x2=31.263，P＜0.001);RASSF1A蛋白表达与淋巴结转移、TNM分期有关(X2=11.498和7.912，P均＜0.05)，而与年龄、性别、肿瘤细胞分化程度和大体分型无关(P均＞0.05)；RASSF1A蛋白阴性表达的ESCC患者5 a生存率低于阳性表达者。结论：RASSF1A阴性表达可能与ESCC的发生发展和预后有关。%Aim: To investigate the expression of RASSF1A protein in human esophageal squamous cell carcinoma (ESCC) tissue. Methods: The expression of RASSF1A in 245 cases of ESCC tissues (observed group) were compared with that in 201 cases of normal esophageal tissues (control group) by immunohistochemical techniques. Its correlation with age, sex,tumor ceil differftiation,lymph nodes metastasis,TNM stage,general classification,and five years survival rate of ESCC patients were analized. Results:Positive expression rate of RASSF1A was 73. % in the control group,and normal expression rate of RASSF1A was 46.9% in the observed group(X2 = 31. 263 , P < 0. 001). The negative expression of RASSF1A were correlated with lymph nodes metastasis and TNM stage (X2 = 11.498,7. 912,P <0. 05) ,but not with age, sex, tumor cell differftiation and general classification (P>0. 05). The five years survival rate of ESCC patients with RASSF1A negative expression was lower than ESCC patients with RASSF1A positive expression. Conclusion: The negative expression of RASSF1A may play an important role in the malignant and progression of ESCC.