河南汉族14个Y-STR基因位点单倍型的遗传多态性

摘要

Aim:To detect 14 Y-STR loci haplotype genetic polymorphism and investigate its application value on legal physical evidence. Methods: PCR, polyacrylamide gel and silver staining banding techniques were used to detect the distribution of 14 Y-STR loci including DYS713, DYS720, DYS635, DYS456, DYS389 Ⅰ , DYS390, DYS389 Ⅱ, H4, DYS460( A7. 1 ),DYS391, DYS448, DYS393 ,DYS439, DYS392 in 203 unrelated individuals of Han nationality from Henan, and the result was used in 2 cases of forensic evidence. Results: A total of 203 haplotypes were detected in 203 samples, haplotype diversity value was up to 1, and individual recognition rate was 0. 999 976. Used in 2 cases of forensic evidence, accurate judgement was got. Conclusion:The 14 Y-STR loci system has high haplotype diversity and discriminating power.%目的:检测河南汉族群体14个Y-STR位点单倍型的遗传多样性,探讨其在法医物证学中的应用价值.方法:应用PCR、聚丙烯酰胺凝胶电泳、银染显带技术,分析DYS713、DYS720、DYS635、DYS456、DYS389Ⅰ、DYS390、DYS389Ⅱ、H4、DYS460(A7.1)、DYS391、DYS448、DYS393、DYS439及DYS392等14个Y-STR位点单倍型在203名河南汉族无关个体中的分布,并用于2例法医物证检案的判定.结果:203例样本中共检出203种单倍型,单倍型多样性高达1,个体识别率为0.999 976.应用于2例法医物证检案,得到准确判断.结论:构建的Y-STR复合体系具有较高单倍型遗传多态性和个体识别力.