八核铜络合物对SGC-7901细胞生长的影响

摘要

Aim:To explore the effect of eight nuclear copper complexes( N-Cu ) on the growth of SGC-7901 cell. Methods:SGC-7901 cells were treated with different concentrations( 3. 125,6. 250,12. 500,25. 000,50. 000,and 100.000 mg/L ) of N-Cu for 24,48,and 72 h, the cell proliferation was detected by MTT assay,and IC50 was calculated. SGC-7901 cells were treated with different concentrations( 12.5 mg/L,25.0 mg/L,50.0 mg/L) of N-Cu for 24,48, and 72 h,cell cycle and apoptosis rate were detected using flow cytometry, and the expression of Caspase-3 protein was detected using Western-blot assay. Results:IC50 of N-Cu to SGC-7901 cells was 73.45,33.71 and 22. 78 mg/L at 24,48 and 72 h. In time-and concentration-dependent manner,N-Cu could inhibit SGC-7901 proliferation, arrested it at G0/G1 phase( Fcomcentration = 3 586.750,Ftime = 418. 133,P <0. 001 ), increased the expression of Caspase-3 protein( F = 55. 133,P < 0. 001 ), and promoted apoptosis( F = 66. 932 ,P <0. 001 ). Conclusion: N-Cu could restrain the cell proliferation by regulating the apoptosis of SGC-7901 cells through inducing the expression of Caspase-3 partly.%目的:研究八核铜络合物(N-Cu)对人胃癌SGC-7901细胞生长的影响,探讨N-Cu抗肿瘤活性的相关机制.方法:体外常规培养人SGC-7901细胞,用不同质量浓度的N-Cu(3.125、6.250、12.500、25.000、50.000、100.000 mg/L)分别处理24、48和72 h后,MTT法检测SGC-7901细胞增殖抑制率,计算IC50.分别用12.5、25.0和50.0 mg/L N-Cu处理SGC-7901细胞,倒置显微镜和扫描电镜下观察细胞形态的改变,流式细胞仪测定SGC-7901细胞周期和凋亡,Western-blot法检测Caspase-3蛋白的表达.结果:N-Cu作用24、48和72 h对SGC-7901细胞的IC50分别为73.45、33.71和22.78 mg/L.随着N-Cu质量浓度的增加和作用时间的延长,扫描电镜下可见细胞微绒毛逐渐消失,胞质收缩,细胞呈球形,早期凋亡细胞形成膜包裹的凋亡小体凸出于细胞表面.N-Cu可剂量依赖性和时间依赖性地抑制SGC-7901细胞的增殖,将其阻滞在G0/G1期(F组间=3 586.750,F时间= 418.133,F交互=224.383,P均<0.001).N-Cu可剂量依赖性地促使SGC-7901细胞发生凋亡(F=66.932,P<0.001),增强Caspase-3蛋白的表达(F=55.133,P<0.001).结论:N-Cu可通过诱导Caspase-3蛋白的表达,参与SGC-7901凋亡的调节,抑制细胞增殖.