紫杉醇肿瘤靶向给药系统对MCF-7细胞的抑制作用观察

摘要

Aim:To prepare targeting drug delivery system NGR-SWCNTs-Paclitaxel and observe its effect on MCF-7 cell.Methods:Single-walled carbon nanotubes loading paclitaxel (SWCNTs-Paclitaxel) and NGR-SWCNTs-Paclitaxel were prepared.The MCF-7 cells were treated with paclitaxel ,SWCNTs-Paclitaxel and NGR-SWCNTs-Paclitaxel labelled with FITC,then phagocytosis test was performed in order to evaluate the intake of MCF-7 cell, cell proliferation was tested by MTT assay, and cell cycle and apoptosis were determined by flow cytometry method .The cells without any treatment was the control.Results:NGR-SWCNTs-Paclitaxel was devoured by MCF-7 cell effectively compared with SWCNTs-Paclitaxel and Paclitaxel.The proliferation inhibition ratio and the apoptosis rate had significant difference among these groups ( P <0.05).The proliferation inhibition ratio and the apoptosis rate of the cells treated by NGR-SWCNTs-Paclitaxel were the highest, and the cells were blocked in G2/M(P<0.05).Conclusion: NGR-SWCNTs-Paclitaxel has been successfully constructed , which could target the MCF-7 cells and inhibit the cell proliferation .%目的：制备紫杉醇肿瘤靶向给药系统NGR-SWCNTs-Paclitaxel ，观察其对MCF-7乳癌细胞的影响。方法：实验分为对照组、Paclitaxel组、SWCNTs-Paclitaxel组和NGR-SWCNTs-Paclitaxel组。用荧光物质FITC标记单壁碳纳米管（ SWCNTs ），采用细胞吞噬实验观察MCF-7细胞对各制剂的体外摄取情况，用MTT法观察各制剂对MCF-7细胞的增殖抑制作用，采用流式细胞术检测细胞周期和凋亡率。结果：MCF-7细胞能够有效吞噬NGR-SWCNTs-Pacli-taxel复合物，未经NGR-SWCNTs修饰的Paclitaxel 较少进入肿瘤细胞。 Paclitaxel、SWCNTs-Paclitaxel 和NGR-SWC-NTs-Paclitaxel组的细胞增殖抑制率、细胞周期和细胞凋亡率差异均有统计学意义（P＜0．05）；NGR-SWCNTs-Pacli-taxel对细胞的增殖抑制作用最强，诱导的细胞凋亡最有效，G2/M期阻滞效果也最显著（P＜0．05）。结论：成功制备的NGR-SWCNTs-Paclitaxel复合物有良好的肿瘤靶向性和乳癌细胞增殖抑制作用。