先天性巨结肠肠壁组织中 L1 CAM 的表达

摘要

目的：探讨先天性巨结肠（HD）肠壁组织中L1CAM的表达情况，从而分析L1CAM与HD的关系。方法：利用免疫组化SP法和RT-PCR法检测48例HD患儿狭窄段和扩张段肠壁组织以及46例正常结肠肠壁组织中L1CAM蛋白和mRNA的表达。结果：HD狭窄段肠壁组织中L1CAM蛋白表达量为（13．1470±0．5198），低于HD扩张段及正常结肠肠壁组织的（16．9618±0．9054）、（17．9083±0．7406）（t配对/t＝27．697、25．346，P均＜0．001）。 HD狭窄段肠壁组织中L1CAM mRNA表达量为（4．3164±0．3870），低于HD扩张段及正常结肠肠壁组织的（5．7178±0．4860）、（6．3443±0．2366）（t配对/t＝19．661、14．237，P均＜0．001）。狭窄段肠壁组织中L1CAM蛋白和mRNA的表达呈正相关（r＝0．968，P＜0．001）。结论：L1CAM蛋白和mRNA的异常低表达可能参与了HD的发生过程。%To investigate the expression of L1CAM in intestinal wall tissues of Hirschsprung disease (HD), and to analyze the relationship between L 1CAM and HD.Methods:SP method and RT-PCR were used to detected the protein and mRNA expression of L1CAM in the stenotic and ectatic intestinal wall tissues of 48 patients with HD and 46 cases of normal colon intestinal wall tissues .Results:The protein expression of L1CAM in the stenotic intestinal wall tissues of HD was (13.147 0 ±0.519 8),which was lower than those of the ectatic intestinal wall tissues of HD (16.961 8 ±0.905 4) and the normal colon intestinal wall tissues (17.908 3 ±0.740 6) (tpaired/t=27.697, 25.346, P<0.001).The mRNA expression of L1CAM in the stenotic intestinal wall tissues of HD was (4.316 4 ±0.387 0),which was lower than those of the ectatic intestinal wall tissues of HD (5.717 8 ±0.486 0) and the normal colon intestinal wall tissues (6.344 3 ± 0.236 6) (tpaired/t=19.661, 14.237, P<0.001).The protein and mRNA expression of L1CAM in the stenotic intestinal wall tissues of HD had the positive relationship (r=0.968, P<0.001).Conclusion:Abnormal low expression of L1CAM mRNA and protein in the stenotic intestinal wall tissues may be involved in the pathogenesis of HD .