番泻叶水提物对3 T3-L1前脂肪细胞增殖、凋亡及脂联素基因表达的影响

摘要

目的:观察番泻叶水提物(FSAE)对3T3-L1前脂肪细胞增殖及凋亡影响,并进一步研究FSAE对3T3-L1前脂肪细胞脂联素mRNA及脂联素启动子荧光素酶活性的影响.方法:体外培养3T3-L1前脂肪细胞,给予不同质量浓度FSAE刺激24 h,MTT法检测细胞增殖,AnnexinV-FITC/PI双染法检测细胞凋亡,Western blot法检测细胞凋亡蛋白Caspase-3、Bax的表达,荧光定量PCR、双荧光素酶报告基因法检测FSAE对3T3-L1脂肪细胞脂联素mRNA和脂联素基因启动子活性的影响.结果:不同质量浓度FSAE对3T3-L1前脂肪细胞的增殖有抑制作用,能够促进细胞凋亡,提高凋亡蛋白Caspase-3、Bax蛋白的表达;同时,FSAE能够增加3T3-L1前脂肪细胞中脂联素mRNA的表达,提高其启动子活性.结论:上述研究结果可能为FSAE抑制肥胖提供分子生物学依据.%Aim:To determine the effect of Folium Sennae aqueous extract ( FSAE) on 3T3-L1 cell proliferation and apoptosis, and further study its effect on 3T3-L1 adiponectin mRNA and adiponectin promoter luciferase activity .Methods:3T3-L1 cells were incubated with different concentrations of FSAE for 24 h, and cell proliferation was determined by MTT method;AnnexinV-FITC/PI double staining was used to detect cells apoptosis;Western blot was used to detect the expres-sions of Caspase-3 and Bax protein;Fluorescence quantitative PCR was used to detect adiponectin mRNA expression ;doub-le fluorescein enzyme report gene method was used to detect the influence of adiponectin gene promoter activity .Results:Different concentration FSAE inhibited 3T3-L1 proliferation,promoted cell apoptosis , improved the expressions of apoptosis related protein Caspase-3 and Bax;At the same time , FSAE improved adiponectin mRNA expression , and enhanced its adi-ponectin promoter activity .Conclusion:This may provide a molecular basis for FASE inhibiting obesity .