青钱柳低聚糖对3 T3-L1前脂肪细胞增殖分化及相关基因表达的影响

摘要

The effect of Cyclocarya paliurus oligosaccharide ( CPO) on the proliferation and differentiation of 3T3⁃L1 adipocytes and the related gene mRNA expression was investigated.CPO was obtained through water solution and alcohol sedimentation,and isolated and purified with D301⁃R macroporous resins and DEAE cel⁃lulose anion exchange resins.The molecular weight was measured with the GPC and MALLS methods.The effect of CPO on the proliferation and differentiation of 3T3⁃L1 adipocytes was studied,and the related expression of PPARγand C/EBPαmRNA were determined by Q⁃PCR.The results showed that CPO consisted of two differ⁃ent components and their weight⁃average molecular weights (Mp) were 3 842 Da and 1 481 Da.CPO promoted the proliferation at the low concentration and restrained it at the high concentration.CPO decreased the expres⁃sion of PPARγand C/EBPαwith significant differences from the control group(P<0.01).The results suggested that hith⁃dosaged CPO restrained the proliferation and differentiation of 3T3⁃L1 adipocytes,which might be re⁃lated with its inhibiting the expression of PPARγ and C/EBPα.%探讨青钱柳低聚糖（ Cyclocarya paliurus oligosaccharide，CPO）对3T3－L1脂肪细胞增殖分化及相关基因表达的影响。采用水提醇的方法得到青钱柳初多糖，经D301－R大孔吸附树脂和DEAE纤维素阴离子交换树脂分离纯化，收集CPO作用于3T3－L1前脂肪细胞，并采用Q－PCR测定相关基因过氧化物酶体增殖物激活受体γ（ peroxisomes proliferator－activated receptor γ， PPARγ）和 CAAT／增强子结合蛋白α（ CAAT／enhancer binding proteinα，C／EBPα） mRNA表达。结果表明：CPO含有2个组分，其重均分子量分别为3842 Da和1481 Da；CPO在低浓度时能促进脂肪细胞的增殖，高浓度时能抑制脂肪细胞的增殖；对脂肪细胞分化的影响较小。 CPO可抑制PPARγ和C／EBPα mRNA表达，与对照组比较差异有极显著性意义（P＜0．01）。表明高浓度的CPO抑制脂肪细胞增殖分化水平，其机制可能与抑制PPARγ和C／EBPα mRNA表达有关。