巴戟天寡糖抗大鼠子宫缺血再灌注损伤作用的观察

摘要

Aim:To investigate the protective effect of morinda officinalis oligose(MOO) on uterine ischemia-reperfusion injury in rats.Methods: A total of fifty Wistar rats were randomly divided into 5 groups: the sham operation group,the uterine ischemia-reperfusion injury model group(model group),the MOO 0.7 g/(kg·d) group,the MOO 1.4 g/(kg·d) group,the MOO 2.8 g/(kg·d) group,and each group had 10 rats.The suture method was used to establish the uterine ischemia-reperfusion injury model.The rats in the sham operation group and the model group were given double steaming water at 10 mL/kg;the rats were given MOO solution accordingly at 10 mL/kg in the three MOO groups.Then the superoxide dismutase(SOD),malondialdehyde(MDA) and high energy phosphates(HEP) in uterine tissue were determined.Results: Compared with the model group,the SOD level was increased significantly in the 3 MOO groups,and the MDA level was decreased significantly(P<0.05);the adenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP) and total adenine nucleotides(TAN) levels were increased significantly in the 3 MOO groups(P<0.05).There were significant differences in the SOD,MDA,ATP,ADP,AMP and TAN levels between the three MOO groups(P<0.05).Conclusion: MOO could improve the antioxidation and energy metabolism of the ischemia-reperfusion injured uteruses,and protect the uterine tissue from ischemia-reperfusion injury.%目的:观察巴戟天寡糖(MOO)对大鼠子宫缺血再灌注损伤的作用.方法:Wistar大鼠50只,随机分为假手术组、子宫缺血再灌注损伤模型组以及3个MOO组[0.7、1.4、2.8 g/(kg·d)],每组10只,采用线栓法制备大鼠子宫缺血再灌注损伤模型,造模前各组进行相应处理:假手术组和子宫缺血再灌注损伤模型组给予10 mL/kg双蒸水,3个MOO组分别给予相应浓度的药液10 mL/kg,实验结束后取出大鼠子宫,并检测组织中超氧化物歧化酶(SOD)、丙二醛(MDA)及高能磷酸化合物(HEP)的水平.结果:与子宫缺血再灌注损伤模型组比较,MOO 0.7 g/(kg·d)组、MOO 1.4 g/(kg·d)组、MOO 2.8 g/(kg·d)组SOD水平升高,而MDA水平降低(P<0.05);MOO 0.7 g/(kg·d)组、MOO 1.4 g/(kg·d)组、MOO 2.8 g/(kg·d)组三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)、总腺嘌呤核苷酸(TAN)水平升高(P<0.05).3个MOO组SOD、MDA、ATP、ADP、AMP、TAN水平两两比较,差异均有统计学意义(P<0.05).结论:MOO对子宫缺血再灌注损伤具有明显保护作用,这可能与其能够改善组织的抗氧化能力和能量代谢有关.