Recombinant glucosidase was used to catalysis biosynthesize of aloe-emodin through aloin. Single factor experiment and uniform design were used to optimize the biosynthesize conditions. As the result,the optimal conditons of aleo-emodin’s biosynthesis were as following:reaction time 4 h,combinant glucosidase concentration 50 U/ mL,the ratio of tert-butanol and water 5: 5(v/ v),reaction temperature 43 ℃ ,and pH value 5. 2. Under those conditions,the highest yield was 92. 18% .%采用生物法，利用重组葡萄糖苷酶催化芦荟苷合成芦荟大黄素，通过单因素试验及均匀试验设计得到了合成芦荟大黄素的最佳工艺条件：反应时间4 h，酶浓度50 U/ mL，叔丁醇：水（v/ v）=5：5，反应温度43℃，pH 值5.2.该工艺条件下芦荟大黄素转化率为92.18%.
展开▼
