以毛竹Phyllostachys edulis花为材料,通过聚合酶链式反应(PCR)克隆技术从毛竹中分离得到1个含完整编码区的cDNA,长603 bp,编码200个氨基酸.命名为PheMADS15(GenBank登记号:KU721916).对PheMADS15进行分析表明,该基因具有典型的MADS-box基因结构域,与拟南芥Arabidopsis thaliana的A类基因AP1编码蛋白的同源性为58.65%.通过实时荧光定量聚合酶链式反应(qRT-PCR)克隆技术检测了PheMADS15在毛竹花芽、 苞片、 颖片、 稃片、 浆片、 雄蕊、 雌蕊和幼胚中的相对表达量.分析表明:PheMADS15基因在毛竹花发育的初期表达量最高,主要在花芽中表达,可能参与毛竹成花转变过程.%As a kind of transcription factors, MADS-box genes play significant roles during floral development, but their identification and functions in Phyllostachys edulis remain unclear. Here, we performed functional analysis of the PheMADS15 gene in Ph. edulis. PheMADS15 cDNA was isolated from Phyllostachys edulis by polymerase chain reaction (PCR) (GenBank accession No. KU721916). This study also used a quantitative real-time, polymerase chain reaction (qRT-PCR) and a homology analysis. Results showed that the gene was 603 bp and encoded a protein of 200 aa, which had a typical MADS-box motif. The homology analysis showed that PheMADS15 shared 58.7% similarity with the floral meristem identity gene AP1 indicating that it belonged to A function genes. The qRT-PCR detected expression of PheMADS15 in the floral bud, glume, lemma, palea, lodicule, stamen, pistil, and young embryo. Additionally, PheMADS15 had the highest expression level in the initial-phase of flower development, especially in the floral bud formation stage. These results suggest that Phe-MADS15 might participate in regulating flower development of Ph. edulis.
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