Compared with submerged fermentation ,the physiological status of yeast during the growth swift in solid-state fermentation caught little attention . The change of intracellular metabolite composition is closely related to physiological status . Metabolome analysis of Saccharomyces cerevisiae in exponential growth and post-diauxic growth with solid-state and submerged fermentation was performed . Principal components analysis of metabolite profiles showed that the metabolic shift between different growth phases was accompanied by complex changes of the central metabolism .The amount of the differential metabolites between the two growth stages were 131 and 117 with solid-state and submerged fermentation ,respectively .The protein synthesis ,amino acid metabolism and Kreb's cycle are the main difference between the two growth stages in both processes of solid-state and submerged fermentation .This study laid a foundation for further elucidation of the metabolic regulation mechanism of yeast in different fermentation processes .%与液态发酵相比,对固态发酵过程中酵母生长期转换前后的生理状态研究较少.细胞内的代谢物组成变化与生理状态密切相关.研究了酿酒酵母固态和液态发酵状态下,指数生长期和二次生长期菌体的胞内代谢组差异.结果表明生长阶段的转换伴随着中心代谢的复杂变化.液态和固态发酵过程中,分别检测到了131种和117种不同生长阶段的差异代谢物.两个生长阶段的主要代谢差异均反映在蛋白质合成、氨基酸代谢和三羧酸循环等代谢途径上,与培养方式无关.本研究为进一步阐明不同发酵方式下酵母的代谢调控机制奠定了基础.
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