脑得生片物质基础的HPLC-DAD-MSn分析

摘要

Objective;To identify the major components of traditional Chinese medicine Naodesheng tablet. Methods; A HPLC-DAD-MS" based method was developed to analyze and identify the major components of Naodesheng tablet. Separation was performed on an Agilent Zorbax SB-C18 column(4. 6 mm ×250 mm,I. D,5 μm) with mobile phase consisting of water with 0.05% formic acid and acetonitrile as gradient eluent at the flow rate of 0. 5 ml · min-1. Results; A total of 43 components were detected, among which 22 were identified by comparing their UV absorption profiles, the information of molecular weights, and structures provided by ESI-MS" with those of available standards and reference data, such as Safflor yellow A, 4'-O-Glucosylpuerarin, 3'-hydroxypuerarin, Genistein-8-C-apiosy ( 1-6 ) glucoside, Puerarin, 6"-O-xylosylpuerarin, 6"-O-apiosylpuerarin, 3'-methoxypuruerarin, 3'-methoxy-6"-o-xylosylpuerarin, Daidzin, Genistin, Pueroside A, Notoginsenoside R,, Ginsenoside Re, Ginsenoside Rg1,Daidzein, Biochanin A, Ginsenoside Rb: ,Ginsenoside Re, Ginsenoside Rb2, Ginsenoside Rb3, Ginsenoside Rd. Conclusion; The proposed method can identify the main components of Naodesheng tablet and provide information for the quality control of this medicine.%目的:明确脑得生片的化学物质基础.方法:采用高效液相-二极管阵列-质谱( HPLC-DAD-MSn)联用技术检测并鉴定其主要化学成分.色谱柱为Agilent Zorbax SB-C18 (4.6 mm×250mm,i.d,5μm),流动相为0.05％甲酸-水和乙腈,流速为0.5 ml·min-1.结果:在脑得生片中共检测到43个化学成分,通过比对紫外吸收图谱、标准品ESI-MSn质谱图提供的分子量及结构式,并结合文献报道,推断鉴定出其中22个化合物,分别为羟基红花黄色素A、4′-O-葡萄糖基葛根素、3′-羟基葛根素、大豆苷元-8-C-芹菜糖基(1-6)葡萄糖苷、葛根素、6″-O-木糖基葛根素、6″-O-芹菜糖基葛根素、3 ′-甲氧基葛根素、3 ′-甲氧基-6″-O-木糖葛根素、大豆苷、5,7-三羟基异黄酮-7-糖苷、葛根苷A、三七皂苷R1、人参皂苷Re、人参皂苷Rg1、大豆苷元、鹰嘴豆素A、人参皂苷Rb1、人参皂苷Rc、人参皂苷Rb2、人参皂苷Rb3和人参皂苷Rd.结论:初步明确了脑得生片的化学物质基础,为进一步提高脑得生片的质量控制标准提供了科学数据.