快速康复外科对肝脏缺血再灌注损伤保护作用的实验研究

摘要

[objective]To observe protective effects of fast-track surgery on hepatic ischemical reperfusion injury.[Methods]60 SPF SD rats are randomly divided into the SHAM,the non FTS and the FTS,also the FTS is divided into FTS with herbal medicine and the general TFS.15 in each group.All the rats are underwent bile duct ligation to be the model of jaundice.In the non FTS&TFS, we induce hepatic ischemical reperfusion injury.In the FTS with herbal medicine we use herbal medicine.At last we detect the ALT,AST,MDA,SOD.[Results]About AST&ALT,there is no difference between the SHAM and the FTS(P＞0.05),but in the others there are(P＜0.05).There is no difference between each group about the MDA(P＞0.05).About the SOD, there isno difference between the SHAM and the general FTS(P＞0.05), but in the others there are(P＜0.05).[Conclusions]FTS will reduce the injury of the hepatic cell & the liver function after the hepatic ischemical reperfusion injury.Also herbal medicine can reduce this kind of injury.%[目的]探究快速康复外科(FTS)对肝脏缺血再灌注损伤的保护作用.[方法]健康SPF级SD大鼠60只,随机分成假手术组、非快速康复外科组及FTS组,FTS组分常规组及中药干预组,每组各15只.所有大鼠均制备为梗阻性黄疸模型,FTS组及非FTS组均进行肝脏缺血再灌注操作.FTS中药组加用中药干预.最后检测天门冬氨酸氨基转移酶(AST)、谷氨酸氨基转移酶(ALT)、丙二醛(MDA)、超过氧化物歧化酶(S0D).[结果]ALT、AST水平相互比较,假手术组与FTS组无明显差异(P＞0.05),与非FTS组比较有差异(P＜0.05);非FTS组与FTS组比较,有差异(P＜0.05);FTS中药组与FTS常规组比较,有差异(P＜0.05).各组间MDA比较无差异(P＞0.05);假手术组与FTS常规组S0D无差异(P＞0.05),其余各组相互比较均有差异(P＜0.05).[结论]FTS的因素干预,降低了黄疸大鼠缺血再灌注损伤后肝细胞和肝功能的损伤程度,同时中药作为FTS组间独立的干预因子,可以更进一步降低这种损伤.