[Objective] To study the mechanism of Curcumin in inhibiting human keratinocyte cell line HaCaT cell proliferation induced by IFN-γ.and lay the experimental foundation for clinical use of curcumin in the treatment of psoriasis. [Methods] MTT assay was used to dectect the proliferation ability of HaC2T cells treated with different concentrations of curcumin(0,1.5,10.20,50,100mol·L-1) and different concentrations of IFN-γ (0,1,5,10,25,50,100 mol·L-1), and the inbition effect of curcumin(20 mol·L-1) DAPT(Notch-1 specific inhibitor, 5 mol·L-1) on the HaCaT cell proliferation induced by IFN-γ(50ng/ml); western blot was used to observe the Hesl and Notch-1protein expression of HaCaT cells influenced by 20 mol·L-1 curcumin and 50ng/ml of IFN-γ. [Results] Curcumin inhibited the proliferation of HaCaT cells in a dose-dependent manner, while IFN-γ promoted the proliferation of HaCaT cells in a dose-dependent manner; 20 mol·L-1 curcumin and 5 mol·L-1 DAPT significantly inhibited the proliferation of HaCaT cells induced by 50ng/ ml. 20 mol · L-1curcumin inhibited the expression of Hesl and Notch-1 protein in HaCaT cells induced by 50ng/ml IFN-γ. [Conclusion] Curcumin can effectively inhibit the proliferation of HaCaT cells, and inhibit the proliferation of HaCaT cells induced by IFN-γ. Notch-1 signaling pathway may play an important role in it.%[目的]研究姜黄素抑制IFN-γ促人角质形成细胞株HaCaT细胞增殖的机制,为临床上姜黄素治疗银屑病奠定基础.[方法]MTT(噻唑蓝)法检测不同浓度姜黄素(0、1、5、10、20、50、100 mol·L-1)和不同浓度IFN-γ(0、1、5、10、25、50、100m ol·L-1)对HaCaT细胞增殖的影响,以及姜黄素(20mol·L-1)、Notch-1特异性抑制DAPT(5 mol·L-1)对IFN-γ(50ng·mL-1)促HaCaT细胞增殖作用的抑制情况；Western blot法检测观察20 mol·L-1的姜黄素、50ng·mL--IFN-γ作用下HaCaT细胞Hes1和Notch-1的蛋白表达情况.[结果]姜黄素浓度依赖性抑制HaCaT细胞增殖,而IFN-γ浓度依赖性促进HaCaT细胞增殖；20 mol· L-1姜黄素及5 mol· L-1DAPT明显抑制50ng·mL-1IFN-γ对HaCaT细胞的促增殖作用,20 mol·L-1姜黄素有效抑制50ng·mL-1 IFN-γ对HaCaT细胞Hes1和Notch-1蛋白的促表达作用.[结论]姜黄素有效的抑制HaCaT细胞增殖,同时可明显抑制IFN-γ时HaCaT细胞促增殖作用,Notch-1信号通路在其中可能发挥重要作用.
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