环孢菌素A体内作用2h对淋巴细胞亚群的影响

摘要

Objective] By detecting the peripheral blood lymphocyte subsets of AML patients before and after taking cyclosporin A, preliminary discussion on making two hours effects of cyclosporin A on lymphocyte subsets in the body. [Methods] Fasting and taking cyclosporin A two hours of the expression of peripheral blood T, B lymphocytes and NK cel were detected by flow cytometry in 43 cases of patients with AML after transplantation. At the same time, application of chemiluminescence detection concentration of cyclosporin A. [Results] The results of cyclosporin A concentration: Fasting group is 125.43 ±65.94 ng·ml-1. Taking two hours group is 564.46 ±258.67 ng·ml-1; Taking two hours group compared with the fasting group, CD3+ cel is significantly reduced and NK cel is significantly increased, but CD4+, CD8+, CD19+cel and CD4+/CD8+ have no obvious change. [Conclusions] Taking cyclosporin A 2 hours can achieve distribution and affect the number of CD3+ cel in the body, but can not affect the B cel . To AML patients after transplantation, in order to achieve the appropriate concentration and keep abreast of the body's immune status of patients, it is necessary to monitor the concentration of cyclosporin A and lymphocyte subsets during their taking cyclosporin A, which helps us determine the prognosis.%[目的]通过对急性髓细胞白血病移植患者服用环孢菌素A(cyclosporin A, CsA)前后外周血淋巴细胞亚群的检测，初步探讨CsA体内作用2h对淋巴细胞亚群的影响。[方法]采用流式细胞术检测43例急性髓细胞白血病移植后患者的空腹及服用CsA 2h的外周血T、B淋巴细胞，NK细胞的表达；同时应用化学发光法检测CsA的浓度。[结果]43例急性髓细胞白血病移植患者空腹CsA浓度为(125.43±65.94)ng·mL-1，服药2h浓度为(564.46±258.67) ng·mL-1；服药2h与空腹相比，CD3+细胞显著下调而NK细胞显著上升，但CD4+、CD8+、CD19+细胞及CD4+/CD8+却无明显变化。[结论]服用CsA 2h即能达到体内分布且影响CD3+细胞的数量，尚不能影响B细胞的数量。对于急性髓细胞白血病患者移植后服用CsA期间，有必要对CsA的浓度及淋巴细胞亚群进行监测以求达到合适的血药浓度，了解患者机体免疫状况，判断预后。