慢性束缚抑郁小鼠痛阈的改变及其机制

摘要

目的 研究慢性束缚抑郁小鼠痛阈的改变并探讨其作用机制.方法 昆明种小鼠随机分为3组:对照(CON)组、慢性束缚(RS)组和慢性束缚一米非司酮(RSM)组.RS和RSM组采用慢性束缚的方法建立抑郁模型,RSM组在每次束缚前30min灌胃给予50mg?kg-1米非司酮溶液,连续9d.9d后测量各组小鼠的糖水消耗百分比和体重,并计算9d体重增量;采用甩尾实验测量小鼠实验第10、11、12天的甩尾潜伏期(TFL).结果 与CON组相比,RS组小鼠的糖水消耗百分比、9d体重增量均显著降低,实验第10、11、12天TFL显著降低,差异有统计学意义(P<0.05或P<0.01).与RS组相比,RSM组小鼠的糖水消耗百分比、9d体重增量显著增加,实验第10、11、12天TFL显著增加,差异有统计学意义(P<0.05或P<0.01).结论 慢性束缚抑郁小鼠痛阈下降,其机制与糖皮质激素受体有关.%Objective To investigate the effects of chronic restraint on the pain threshold of depression mice and its mechanism.Methods Kunming mice were randomly divided into 3 groups:a control(CON)group,a chronic restraint(RS)group and a chronic restraint-mifepristone(RSM)group.A depression model of mouse was established through chronic restraint in the RS and RSM groups.The RSM group was intragastrically administered with 50 mg?kg-1 mifepristone for 9 days.Then,the sucrose preference percentage and weight were measured in each group,and the weight gain was calculated.The tail-flick latency(TFL)was measured on Days 10,11 and 12.Results Compared with the CON group,the RS group showed remarkable decreases in the sucrose preference percentage and weight gain within 9 days,and TFL on Days 10,11 and 12(P<0.05 or P<0.01).Compared with the RS group,the RSM group showed remarkable increases in the sucrose preference percentage and weight gain within 9 days,and TFL on Days 10,11 and 12(P<0.05 or P<0.01).Conclusions The pain threshold of depression mice induced by chronic restraint is decreased,which may be related to glucocorticoid receptor.