不同剂量乙醇对地西泮及氯胺酮用药小鼠学习记忆的影响

摘要

Objective To observe the effects of different doses of ethanol on learning and memory in mice with dia-zepam and ketamine administation. Methods In a stratified and random block design, 80 mice were divided into 10 groups (n=S each): saline + diazepam (group A) ; 10% ethanol + diazepam (group B); 20% ethanol + diazepam (group C); 40% ethanol + diazepam (group D); saline + ketamine (group E); 10% ethanol + ketamine (group F) ; 20% ethanol + ketamine (group G); 40% ethanol + ketamine (group H); saline + diazepam + ketamine (group I); 10% ethanol + diazepam + ketamine (group J). The step-through test was performed to observe latency and error times in each group at 1 , 24 and 48 h after drug administration. Results Compared with group A , the error times in group D decreased , but the mice of group D was in a state of lethargy , indicating that ethanol inhibited the cen-tral nervous system highly. Compared with group B , the latency in group C decreased and error times increased (P<0.05) , indicating that with the increase of the concentration of ethanol , the inhibition effect of ethanol on learning and memory in mice with diazepam administration enhanced . Compared with group E , the latency in group F and group G de-creased (P<0.05) , indicating that ethanol could enhance the inhibition effect of learning and memory in mice with ket-amine administration. Compared with group C, the error times in group G decreased and the latency increased (P<0.05) , indicating that the inhibition eliect of ethanol on learning and memory in mice with diazepam administration was stronger than ketamine. Conclusion The inhibition effect of 20% ethanol on learning and memory in mice with diaze-pam administration is stronger than ketamine.%目的 研究不同剂量乙醇对地西泮和氯胺酮用药小鼠学习记忆的影响.方法 按分层随机区组设计将80只小鼠分为10组(每组n=8):生理盐水+地西泮(A组);10%乙醇+地西泮(B组);20%乙醇+地西泮(C组);40%乙醇+地西泮(D组);生理盐水+氯胺酮(E组);10%乙醇+氯胺酮(F组);20%乙醇+氯胺酮(G组);40%乙醇+氯胺酮(H组);生理盐水+地西泮+氯胺酮(I组);10%乙醇+地西泮+氯胺酮(J组).用避暗实验测定各组用药后1、24、48 h的潜伏期和错误次数.结果 与A组相比,D组错误次数减少(P<0.05),但D组小鼠处于昏睡状态,表明乙醇对中枢高度抑制.与B组相比,C组潜伏期缩短、错误次数增多(P<0.05),表明随着乙醇浓度的增高,乙醇对地西泮用药小鼠学习记忆的抑制作用增强.与E组相比,F组和G组潜伏期缩短(P<0.05),表明乙醇可以增强氯胺酮对小鼠学习记忆的抑制作用.与C组相比,G组错误次数减少、潜伏期延长(P<0.05),表明乙醇对地西泮用药小鼠学习记忆的抑制作用强于氯胺酮.结论 20%浓度的乙醇对地西泮用药小鼠学习记忆的抑制作用强于氯胺酮.