长期服用辛伐他汀对机械通气肺损伤大鼠肺湿/干重比与蛋白含量的影响

摘要

目的 对长期服用辛伐他汀大鼠进行机械通气造成急性肺损伤,观察大鼠肺组织病理学变化、湿/干重比(W/D)及蛋白含量的变化,探讨长期服用辛伐他汀对机械通气致急性肺损伤、肺水肿及肺泡通透性的影响.方法 32只雄性SD大鼠随机分为4组(n=8):对照组(A组)不做任何处理；机械通气组(B组)行机械通气,潮气量7 ml/kg;辛伐他汀组(C组)进行辛伐他汀(10 mg· kg-1·d-1)灌胃处理4周；辛伐他汀+机械通气组(D组)辛伐他汀灌胃4周后行机械通气,潮气量7 ml/kg.B组与D组行机械通气4h后处死大鼠,收集肺组织,苏木精-伊红染色光镜下观察病理学改变,测定肺组织W/D比及蛋白含量.结果 与A组相比,B组大鼠肺组织损伤明显,W/D与蛋白含量均明显增高(P＜0.01)；C组各指标无明显变化.与B组相比,D组肺组织病理损伤明显减轻,W/D及蛋白含量明显降低(P＜0.01).结论 长期服用辛伐他汀可以明显降低肺血管与肺泡的通透性,减轻机械通气所致的急性肺损伤、肺水肿.%Objective To investigate the impact of long-term use of simvastatin on wet-to-dry weight ratio (W/D) and protein content in ventilator-induced lung injury in rats.Methods Thirty-two male SD rats were randomly divided into four groups (n =8 each):group A received room air without mechanical ventilation; group B received tidal volume of 7 ml/kg with mechanical ventilation for 4 h; group C:animals were orally administered with 10 mg/(kg · d)simvastatin for 4 weeks; group D:animals were orally administered with 10 mg/(kg · d) simvastatin for 4 weeks and then received tidal volume of 7 ml/kg with mechanical ventilation for 4 h.After 4 h of ventilation,the rats were sacrificed and the lung tissue were collected and stored with correct methods.Pathological changes in the lung was observed by microscope.The measured indexes included W/D of lung and the levels of protein content in lung tissue.Results Pathological damage of lung tissue in group B was significanly more severe than in group A.The levels of protein content in lung tissue and W/D in group B were significantly higher than in group A (P ＜ 0.01).Compared with group B,those indexes in group D were significantly decreased (P ＜ 0.01).Conclusion Long-term use of simvastatin can significantly reduce pulmonary vascular and alveolar permeability and lighten pulmonary edema caused by (ical) ventilation in acute lung injury.