n-6-3多不饱和脂肪酸对子宫内膜癌Ishikawa细胞增殖的影响

摘要

Objective To explore the effects and mechanisms of different n-6-3 polyunsaturated fatty acids (PUFAs) ratio on endometrial carcinoma Ishikawa cells . Methods Ishikawa cells were treated with n-6 PUFAs,or n-3 PUFAs,or the mixture of n -6-3 PUFAs at ratio of 1:1 and 10:1. The cell proliferation capacity was detected by MTT,and the expressions of mTOR,VEGF mRNA were tested by RT - PCR respectively. Results n - 6 PUFAs and 10:l n -6 -3 PUFAs obviously promoted cell proliferation and up -regulated the expression of mTOR,VEGF mRNA. n-3 PUFAs and 1:1 n-6-3 PUFAs greatly repressed cell proliferation and down-regulated mTOR,VEGF mRNA expression. There was significant correlation between mTOR and VEGF in Ishikawa cell (r=0.94,P <0.05). Conclusion n-6-3 PUFAs at different ratios has different effects in endometrial carcinoma Ishikawa cells through working on mTOR signal to regulate VEGF expression probably .%目的 探讨n-6-3多不饱和脂肪酸(PUFAs)对子宫内膜癌Ishikawa细胞增殖及细胞中哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响.方法用不同比例的n-6-3 PUFAs处理细胞,MTT法检测细胞增殖能力变化;RT-PCR检测细胞mTOR、VEGF的mRNA表达情况.结果单纯n-6 PUFAs和10:1 n-6-3 PUFAs能够明显促进Ishikawa细胞增殖,mTOR、VEGF的mRNA表达增加(P<0.05);单纯n-3 PUFAs和1:1 n-6-3 PUFAs则显著抑制细胞增殖,同时使VEGF的mRNA表达下降(P<0.05);mTOR与VEGF mRNA的表达呈正相关(r=0.94,P<0.05).结论 n-6-3 PUFAs可能通过影响mTOR信号调节VEGF表达,从而对Ishikawa细胞增殖产生影响.