Objective To study the inhibitory activity of flavonoid extracts and monomer glabridin from Xinjiang Glycyrrhiza glabra L (GgL) towards human Bel‐7402 hepatocarcinoma cells .Methods General flavonoid extract (GF‐A) was prepared from GgL root within ethanol extraction ;the extract GF‐A was purified within polyamide column chromatography for obtaining fine total flavonoids (GF‐B) ;monomer glabridin (Gb) was isolated from the extract GF‐B ;human Bel‐7402 hepatocarcinoma cells were respec‐tively treated with these samples ,and the cell viability was determined by MTT method .Results Three flavonoid samples :GF‐A (flavonoid content 31 .18% ) ,GF‐B (52 .5% ) and monomer glabridin were char‐acterized ;these substances showed significant inhibitory activity towards human Bel‐7402 cancer cell pro‐liferation .At the concentration range of 25 ~ 250 μg/mL ,their maximum inhibitory activity was up to 67 .92% ,84 .28% and 90 .11% ,respectively .Conclusion The human Bel‐7402 hepatocarcinoma cells have certain sensitivity to the anticancer mechanism of flavonoid extracts of GgL ;glabridin may be one of the active flavonoid component in this plant with anticancer potency .%目的：探讨新疆光果甘草（Glycyrrhiz a glab ra L）总黄酮及单体成分光甘草定（glabridin ，Gb）对人肝癌 Bel‐7402细胞增殖的抑制活性。方法采用乙醇提取法从光果甘草制备总黄酮提取物（GF‐A ）；经聚酰胺富集法精制而制备黄酮含量高于50％的标准化提取物（GF‐B）；从 GF‐B 中纯化制备单体成分光甘草定（Gb）；以人肝癌Bel‐7402细胞株作为体外模型，用 M TT 法测定各样品对肝癌细胞增殖的抑制活性。结果制备和鉴定3种提取物：GF‐A（总黄酮含量31．18％）、GF‐B（含量52．5％）和 Glabridin 单体；3种物质对人肝癌 Bel‐7402细胞的增殖显示明显的抑制活性，其浓度在25～250μg／mL 范围内对肿瘤细胞的最高抑制率分别达67．92％、84．28％和90．11％。结论人肝癌 Bel‐7402细胞对光果甘草总黄酮的抗癌活性具有一定的敏感性；单体 Glabridin 可能是光果甘草总黄酮中抗癌活性较高的有效成分之一。
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