摘要：
[目的]确定光果甘草种子萌发的最适温度和最佳盐分条件,为甘草资源的人工种植和盐碱地资源的开发利用提供理论依据.[方法]采用恒温培养箱+培养皿滤纸萌发法,研究了不同温度(15,20,25,30,35°C)及不同浓度(0(CK),50,100,150,200,250,300,350 mmol/L) NaCl和Na2 SO4对光果甘草种子萌发的影响.[结果](1)光果甘草种子萌发的最适温度为30°C,亚适宜温度为25°C,高于30°C时,发芽率随着温度的升高而逐渐越低.(2)低浓度(50～100 mmol/L)2种盐处理下,光果甘草种子发芽率、发芽势和发芽指数均高于对照,且盐害指数为负值;在高浓度(≥150 mmol/L)条件下,2种盐处理光果甘草种子的发芽率、发芽势和发芽指数均低于对照,并随着浓度的增高而降低,盐害指数随之升高.(3)相同浓度下NaCl处理光果甘草种子发芽率、发芽势和发芽指数均高于Na2SO4,盐害指数则相反;低浓度NaCl对光果甘草种子萌发的促进作用优于Na2SO4,高浓度Na2SO4胁迫对光果甘草种子萌发的抑制作用强于NaCl.[结论]在人工种植或培养幼苗过程中,光果甘草种子萌发的最适温度为25～30°C,最适盐分条件为50～100 mmol/L NaCl溶液处理.%[Objective] This study determined the optimal temperatue and salt condition for seed germination of Glycyrrhiza glabra L.to provide theoretical basis for the large-scale artificial plant of licorice resources and use of saline lands.[Method] Petri-dish and filter-paper in incubator methods were used to study the influence of different temperatures (15,20,25,30,and 35 °C) and salt (NaCl and Na2SO4) concentrations (0 (CK),50,100,150,200,250,300,and 350 mmol/L) on seed germination of Glycyrrhiza glabra L.[Result] (1) The optimal temperature for seed germination was 30C,the sub-optimal temperature was 25 °C,and the germination rate decreased with increase of temperature when temperature was higher than 30 °C.(2) Under the treatments of lower salts concentrations (50-100 mmol/L),the seed germination rate,germination potential and germination index were higher than control,and salt injury index was negative.Under higher concentrations (≥ 150 mmol/L),seed germination rate,germination potential and germination index were lower than control and they decreased with the increase of salt concentration.Salt injury index was positive and increased with salt concentration.(3) Under same concentrations,the seed germination rate,germination potential and germination index of NaCl treatments were higher than those of Na2SO4,while salt injury index was opposite.Low salt concentrations (50 and 100 mmo/L) were beneficial for seed germination,and the promoting effect of NaCl was better than Na2SO4.High concentrations Na2SO4 had larger inhibitory effect than NaCl.[Conclusion] During the artificial cultivation or seedling cultivation of Glycyrrhiza glabra L.,the optimal temperature was 25-30 °C and the best salt treatment was 50-100 mmol/L NaCl.
