转化生长因子-β1、结缔组织生长因子与胆道闭锁患儿肝硬化程度相关性研究

摘要

Objective To investigate the correlation of the transforming growth factorβ1 (TGF-β1 ),connec-tive tissue growth factor (CTGF)and degree of biliary atresia (BA)in children patients.Methods Using enzyme-linked assay (ELISA method)to detect the expression of TGF-β1 and CTGF in serum of biliary atresia and control groups and immunohistochemistry to detect the expression of TGF-β1 and CTGF in liver tissue of the groups.Results The expression level of TGF-β1 and CTGF in biliary atresia group was signif-icantly higher than control group (P <0.05).The expression level of TGF-β1 and CTGF in serum of before surgery was higher than that of post-operative in the biliary atresia group (P <0.05).With the gradually increased hepatic fibrosis,the expression level of serum TGF-β1 and CTGF were gradually higher in biliary atresia group before surgery,which was showing positive correlation (r =0.712,P =0.021 for TGF-β1 ;r =0.581,P =0.039 for CTGF vs hepatic fibrosis).The expression of TGF-β1 and CTGF of children with biliary atresia in liver tissue were higher than control group that the difference was statistically significant (P <0.05).With gradually increased of hepatic fibrosis,TGF-β1 and CTGF expression levels in liver in children with biliary atresia were gradually increased,which showed positive correlation (r =0.624,P =0.026 for TGF-β1 ;r =0.512,P =0.041 for CTGF vs hepatic fibrosis).Conclusion There were higher ex-pressions of TGF-β1 and CTGF in serum and liver tissue of children with biliary atresia.TGF-β1 and CTGF were closely involved in the formation of liver fibrosis in children with biliary atresia,which could be po-tential pre-judgment indicators of liver fibrosis for biliary atresia children.%目的：探讨转化生长因子-β1（Transforming growth factor-β1，TGF-β1）、结缔组织生长因子（Connective tissue growth factor，CTGF）与胆道闭锁（Biliary atresia，BA）患儿肝硬化程度的相关性。方法运用酶联吸附试验（ELISA 法）检测胆道闭锁组和对照组患儿血清中 TGF-β1及 CTGF 的表达。运用免疫组化法检测胆道闭锁组和对照组患儿肝脏组织中 TGF-β1及 CTGF 的表达。结果胆道闭锁组患儿血清中 TGF-β1、CTGF 的含量明显高于对照组（P ＜0．05）。胆道闭锁组患儿术前血清中 TGF-β1、CTGF 的表达水平高于术后的表达水平（P ＜0．05）。随着肝纤维化的逐渐加重，胆道闭锁组患儿术前血清中 TGF-β1、CTGF 的表达水平逐渐升高，术前胆道闭锁患儿血清中 TGF-β1、CTGF 水平与肝纤维化呈正相关（r ＝0．712，P ＝0．021；r ＝0．581，P ＝0．039）。胆道闭锁组患儿肝脏组织中 TGF-β1、CTGF 的表达高于对照组，差异有统计学意义（P ＜0．05）。随着肝纤维化的逐渐加重，胆道闭锁组患儿肝脏组织中 TGF-β1、CTGF 的表达水平逐渐升高，且 TGF-β1、CTGF 表达水平与肝纤维化呈正相关（r ＝0．624，P ＝0．026；r ＝0．512，P ＝0．041）。结论TGF-β1、CTGF 在胆道闭锁患儿血清中有较高表达， TGF-β1、CTGF 在胆道闭锁患儿肝脏组织中的也有较强的阳性表达。TGF-β1、CTGF 密切参与胆道闭锁患儿肝纤维化的形成，TGF-β1、CTGF 可以作为胆道闭锁患儿肝纤维化的潜在预判定指标。