破布木果提取物对小鼠脾淋巴细胞的增殖作用

摘要

Objective To investigate the proliferative effects of extracts from Cordia dichotoma seeds on spleen lymphocytes of mice.Methods Detect the effects of the extracts with different concentrations from Cordia dichotoma seeds on mouse spleen lymphocytes by concanavalin A (ConA)and lipopolysaccharide (LPS)in vitro treatment;Detect the proliferation ability of lymphocyte by MTT assay.Results The ex-tracts of Cordia dichotoma seeds in the range of 2.69-86 mg/mL had a synergistic effect in vitro on the mouse spleen lymphocytes proliferation induced by ConA and LPS;polysaccharides of Cordia dichotoma seeds in the range of 21.50-86 mg/mL had synergistic effects in vitro on the spleen lymphocyte prolifera-tion induced by ConA;polysaccharides in the range of 10.75-86 mg/mL had synergistic effects in vitro on the spleen lymphocyte proliferation induced by LPS;rosmarinic acid standard,used as the positive control drug,had a synergistic effect in vitro on the mouse spleen lymphocytes proliferation induced by ConA and LPS.Conclusion This primary study showed that the extracts from Cordia dichotoma seeds,polysaccha-rides of Cordia dichotoma seeds and rosmarinic acid standards had some immunomodulatory effects.%目的：研究破布木果提取物对小鼠脾淋巴细胞的增殖作用。方法不同浓度的破布木果提取物对刀豆蛋白 A（concanavalin A，ConA）、脂多糖试剂（LPS）体外处理的小鼠脾淋巴细胞的作用；采用 MTT法测定淋巴细胞的增殖能力。结果破布木果提取液在2．69～86 mg／mL范围内，均与 ConA、LPS诱导的小鼠脾淋巴细胞的增殖在体外具有协同作用；破布木果多糖在21．50～86 mg／mL范围内，与 ConA诱导的小鼠脾淋巴细胞的增殖在体外具有协同作用；在10．75～86 mg／mL范围内，与 LPS诱导的小鼠脾淋巴细胞的增殖在体外具有协同作用；迷迭香酸标准品作为药物的阳性对照，均与 ConA、LPS诱导的小鼠脾淋巴细胞的增殖在体外具有协同作用。结论初步明确破布木果提取液、破布木果多糖、迷迭香酸标准品具有一定的免疫调节作用。