肺结核患者血清中 sPD-1和 sPD-L1与细胞因子的相关性研究

摘要

Objective To detect the level of soluble programmed death 1(sPD-1)and soluble programmed death ligand 1 (sPD-L1),IFN-γ and TNF-α in the serum of patients with pulmonary tuberculosis.Met-hods 40 patients with pulmonary tuberculosis and 35 healthy people were enrolled,followed by divided into two groups.Namely,tuberculosis group and healthy control group.The serum level of sPD-1 and sPD-L1 were detected by enzyme-linked immunosorbent assay (ELISA).The levels of IFN-γ and TNF-αwere detected by cytokine bead array (CBA).Pearson correlation was used to analyze the correlation be-tween sPD-L1,IFN-γ and TNF-αlevels in serum of patients with pulmonary tuberculosis.Results Com-pared with the control group,no significant change was observed in terms of level of sPD-1 in the sera;sPD-L1 was found to be significantly increased whereas both IFN-γand TNF-αwas shown to be markedly decreased in the sera of patients with pulmonary tuberculosis (P < 0.05).Pearson analysis showed that there was a significantly inversed correlation between sPD-L1 and IFN-γ,TNF-α(P <0.05).Conclusion sPD-L1 was shown to be remarkably increased whereas IFN-γ and TNF-α were presented to be pro-nouncedly decreased in the sera of patients with pulmonary tuberculosis,which might be involved in the development of Pulmonary tuberculosis..%目的：探讨肺结核患者血清中可溶性程序性死亡受体1（sPD-1）及其配体可溶性程序性死亡配体1（sPD-L1）、干扰素γ（IFN-γ）和肿瘤坏死因子α（TNF-α）的水平及其相关性。方法收集40例肺结核患者（肺结核组）和35例健康体检者（对照组）外周血，采用酶联免疫吸附试验（ELISA）检测血清中 sPD-1、sPD-L1水平；流式液相多重蛋白定量技术（CBA）检测血清中 IFN-γ、TNF-α水平，Pearson 法分析肺结核组血清中 sPD-L1与 IFN-γ、TNF-α的相关性。结果与对照组相比，肺结核组血清中 sPD-1水平无明显变化，sPD-L1水平明显增高，IFN-γ和 TNF-α水平明显下降；Pearson 分析显示，sPD-L1与 IFN-γ、TNF-α均为负相关（P ＜0．05）。结论肺结核患者血清中 sPD-L1水平增高，IFN-γ、TNF-α水平降低，可能与肺结核发病有关。