远程缺血预处理和远程缺血后处理在兔肺移植缺血-再灌注损伤保护作用中的比较

摘要

Objective To compare the effects of two methods of remote ischemic preconditioning and remote ischemic postprocessingon ischemia reperfusion injury after lung transplantation of New Zealand rabbits.Methods Rabbit models of allograft lung transplantation were established in 48 New Zealand rabbits,and then they were randomly divided into ischemia-reperfusion group (IR),remote ischemic preconditioning group (RIPC),and remote ischemic postprocessinggroup (RIPOST).The oxygen partial pressure (PaO2)reperfusion after transplantation at 60 min,120 min were monitored.The pathological changes,serum superoxide dismutase (SOD),malondialdehyde (MDA),nitric oxide (NO),tumor necrosis factor alpha (TNF-α) contents and lung wet/dry weight ratio (W/D) were determined.Results The rabbit model of lung transplantation was successfully established,IR group and RIPOST model wereestablished by 75％,and RIPC for 87.5％;PaO2 in RIPC group was the highest;compared with the group IR and RIPOST,the pathological changes,SOD,MDA,NO,TNF-α and W/D in group RIPC were better.Conclusion Remote ischemic preconditioning has obvious protective effects on ischemia-reperfusion injury in rabbit lung transplantation.%目的 比较远程缺血预处理和远程缺血后处理这两种方法对新西兰大白兔肺移植术中缺血再灌注损伤的保护作用.方法 对48只新西兰大白兔建立异体兔肺移植模型,随机分为缺血再灌注组(IR组)、远程缺血预处理组(RIPC组)、远程缺血后处理组(RIPOST组).监测移植再灌注后60、120 min氧分压(PaO2)的变化,观察各组动物肺组织病理改变,检测血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)含量和肺湿/干重比(W/D).结果 成功建立了兔肺移植模型,IR组和RIPOST组成模率为75％,RIPC成模率达87.5％;PaO2改变RIPC组最高;病理变化、SOD、MDA、NO、TNF-α和W/D比较得出RIPC对缺血再灌注损伤的保护作用较好.结论 远程缺血预处理对兔肺移植缺血再灌注损伤保护作用较明显.