TYR基因多态性与哈萨克羊毛色相关的研究

摘要

In this study,a total of 216 DNA samples were obtained from 2—3 years old Kazakh sheep within 4 different types of coat color in Tekes County in Ili Kazakh Autonomous Prefecture of Xinjiang,a-mong which,51 were black coat sheep,50 were brown gray sheep,59 were gray sheep and 56 white coat sheep.The main purpose of this study was to analyze the effect of TYR gene polymorphisms on coat color of Kazakh sheep by using PCR-SSCP and sequencing methods.The results showed that three unique SSCP banding patterns were observed (named as AA,AB and BC)in TYR gene.In white and gray coat popula-tions,AB genotype was the dominant genotype,while AA genotype was the dominant genotype in the brown and black coat population.In this population,A allele was the dominant allele in the 4 types of coat color.Theχ2 test results showed that the genotype frequency of TYR gene was not significantly different in the 4 kinds of wool sheep (P >0.05).%采集新疆伊犁哈萨克自治州特克斯县4种不同被毛颜色2~3周岁的216只哈萨克母羊血样,其中黑色被毛羊51只,棕色被毛羊50只,青灰色59只,白色被毛羊56只.运用PCR-SSCP及测序方法对TYR基因多态性及其对哈萨克羊毛色的影响进行分析.结果表明,TYR基因存在3种基因型:AA、AB和BC型.在白色和青灰色被毛群体中,AB基因型为优势基因型.在棕色和黑色被毛群体中,AA基因型为优势基因型.在此群体中A等位基因为4种毛色的优势等位基因.经χ2检验,TYR基因的基因型频率在4种毛色羊中的分布差异不显著(P>0.05).