人乳头瘤病毒16型(湖北株)E7蛋白在 NIH/3T3细胞中的表达和定位

摘要

Objective The eukaryotic plasmid pL(E7-HB)SN with HPV16 E7gene(Hubei strain) was transfected into NIH/3T3 cells for transient expression.Methods The recombinant plasmid was transfected into NIH/3T3 by calcium phosphate-DNA coprecipitation.The expression of E7 gene was examined by RT-PCR and the indirect immunofluorescence.Results It was found that the E7 gene could transcript and express the E7 protein efficiently. And the E7 protein could be seen predominantly in cytoplasm,but it was few in nucleus.Conclusion This phenomenon may be correlated with the mutation of E7 gene.%目的 将带有人乳头瘤病毒16型(湖北株)E7基因的真核表达载体pL(E7-HB)SN导入NIH/3T3细胞进行瞬时表达。方法 重组质粒转染3T3细胞通过DNA-磷酸钙共沉淀法；E7基因表达的检测采用RT-PCR和间接免疫荧光实验。结果 E7基因导入真核细胞后能有效转录并表达E7蛋白，通过免疫荧光染色可看到E7蛋白主要定位在胞浆中，细胞核中也有，但量较少。结论 这可能和湖北株HPV16"E7基因发生突变有关。