肿瘤转移抑制基因-1在食管鳞癌组织及食管癌EC109细胞中的表达及意义

摘要

Objective To study the expression and significance of tumor metastasis suppressor gene-1(TMSG1) in esophageal squamous cell carcinoma (ESCC) and EC109 cells.Methods Immunohistochemistry S-P method was used to examine the expression of TMSG-1 protein in 136 cases of ESCC and 37 cases of normal esophageal mucosa.We analyzed the relationship between TMSG-1 and clinicopathological data of ESCC patients.EC109 cells were treated with 3 μg/mL of cisplatin (CDDP) in vitro for 24 h (the intervention group) and the control group was set up at the same time.The proliferation-inhibitory capability was analyzed with MTT assay.RT-PCR was used to examine the expression of TMSG-1 in the intervention group and the control group.Results The positive rate of TMSG-1 in ESCC and normal esophageal mucosa was 52.2％ (71/136) and 94.6％ (35/37),respectively.The expression of TMSG-1 in ESCC was significantly lower than that in normal esophageal mucosa (P＜0.05).The expression of TMSG-1 was related to TNM stage,differentiation degree and lymph node metastasis (P＜0.05).After EC 109 cells were treated with CDDP for 24 h,the proliferation inhibition rate was increased significantly compared with the control group (P＜0.01).RT-PCR results showed that the expression of TMSG-1 in the cells of the intervention group was significantly higher than that in the control group (P＜ 0.01).Conclusion The abnormal expression of TMSG-1 may play a role in the development and metastasis of ESCC.Examination of TMSG-1 may be useful for making diagnosis and guiding clinical therapy of ESCC.%目的 探讨肿瘤转移抑制基因-1 (TMSG-1)在食管鳞癌(ESCC)组织及食管癌EC109细胞中的表达及意义.方法 采用SP免疫组织化学染色法检测136例ESCC及37例正常食管黏膜组织中TMSG-1蛋白的表达情况,分析TMSG-1与ESCC患者临床病理指标的关系;培养人食管癌EC109细胞并用常用化疗药物顺铂(CDDP)干预,同时设对照组,采用MTT法检测细胞增殖抑制率,半定量RT-PCR法检测细胞TMSG-1的表达情况.结果 TMSG-1在ESCC和正常食管黏膜的阳性率分别为52.2％(71/136)、94.6％(35/37),差异具有统计学意义(P＜0.05).TMSG-1的异常表达与ESCC组织学分级、患者的TNM分期、淋巴结转移情况相关.顺铂干预组EC109细胞的增殖抑制率较对照组显著增高,差异具有统计学意义(P＜0.01).RT-PCR结果显示,干预组EC109细胞TMSG-1的mRNA表达明显高于对照组(P＜0.01).结论 TMSG-1的异常表达与ESCC的发展及转移相关,可作为较为理想的肿瘤标志物辅助诊断并指导临床治疗.