中菏1号杨树上无柄三毛孢的首次报道

摘要

In order to study a fungal diversity on Populus deltoides ‘Zhong He 1 ’ , sap wood tissues were plated and incubated on PDA in July 2009.After purified culturing of the endophytic fungus JSNL 1104 strain, this fun-gus was identified as Robillarda sessilis by using both morphological characters and molecular biological technique . It characterized by pycnidial black , ostiole central , 293.4~616.5 μm ×261.3~464.6 μm, conidiophore ab-sent, conidiogenous cells enteroblastic , phialidic, 7.3~9.2μm ×3.2~4.0μm.2-cells, hyaline, 10.4~12.8μm ×2.4~2.8 μm, and with 2~3 apical appendages.The host range of this fungus indicated that P.deltoides‘Zhong He 1’ is a new host and it is the first report of this fungus on Populus.%为了研究中菏1号杨树上的真菌，2009年7月，对杨木边材进行了组织分离，得到了内生菌JSNL1104菌株。通过纯化培养，采用形态特征和分子生物技术相结合的方法对该菌进行了比对，鉴定为无柄三毛孢，它的孢子器黑色，有孔口，293.4～616.5μm ×261.3～464.6μm。分生孢子梗缺。产孢细胞瓶梗状，7.3～9.2μm ×3.2～4.0μm。分生孢子双细胞，无色，10.4～12.8μm ×2.4～2.8μm，顶端具有2～3根附属丝。根据该菌的寄主范围，确认中菏1号杨树是无柄三毛孢的新寄主。此菌在杨树上属于首次报道。