紫皮石斛上灰葡萄孢的分离和鉴定

摘要

For understanding the relationship between Dendrobium devonianum and fungal on it , tissue isolation were conducted for root , stem and leaf in both diseased and healthy Dendrobium devonianum, and a total 306 fun-gal strains were obtained .Among these fungal strains , the largest number was Botrytis cinerea, which accounted for 23.4 %. After purified culturing , the Botrytis cinerea were identified by means of combining morphological characters with molecular biological techniques .Based on the the fact of host range and distribution range of Botry-tis cinerea, this study showed that D.devonianum is a new host for Botrytis cinerea and this was the first report that D.devonianum could be host of Botrytis cinerea.%为了研究紫皮石斛上的真菌，对紫皮石斛染病和健康根、茎和叶片进行了组织分离，共得到306个真菌菌株，其中灰葡萄孢出现数量最多，占23.4％。通过纯化培养，并采用形态特征和分子生物技术相结合的方法对灰葡萄孢进行了鉴定。根据该菌的寄主范围和分布地区，结果表明，紫皮石斛是灰葡萄孢的新寄主，此菌在紫皮石斛上发生属于首次报道。