铜对海菜花种子萌发及幼苗生长的影响

摘要

Using nutrient solution culture method, the effects of Cu2+on seed germination, seeding growth of Otte-lia acuminatas were studied.The results showed that effect of exogenous Cu2+within the concentration varied from 0.1 to 10 mg/L on seed germination rate of Ottelia acuminatas showed no significant difference compared with con-trol ( p>0.05 ) , while for the inhabitation of germination potential , germination index and vital index , there was significant difference compared with control ( p<0.05 ) in higher concentration .Cu2+had an inhibitory effect on chlorophyll and carotinoid content of Ottelia acuminatas, and there was significant difference compared with control (p<0.05 ).With increasing concentration of Cu2+, the activities of superoxide dismutase ( SOD), peroxidase ( POD) , soluble protein contents of seedling showed increasing at beginning and then decreasing; At the same time, the activity of catalase (CAT) was consistently decreasing, and there was significant difference compared with control ( p<0.05 ) . This study could provide certain theoretical support for the natural Ottelia acuminatas conservation .%采用营养液培养法，研究了不同铜离子（Cu2＋）浓度（0mg／L、0畅1mg／L、0畅2mg／L、0畅5mg／L、1mg／L、2mg／L、5mg／L、10mg／L）对海菜花种子萌发及幼苗生长指标及部分生理指标变化的影响。结果表明，各浓度处理（0畅1～10mg／L） Cu2＋对海菜花种子发芽率与对照均无显著差异（p＞0畅05）；海菜花种子的发芽势、发芽指数与活力指数整体表现出随Cu2＋处理浓度的增加呈下降趋势，当Cu2＋处理浓度较高时，与对照差异显著（ p＜0畅05）。 Cu2＋明显降低海菜花叶片中叶绿素a、 b及类胡萝卜素含量，且与对照差异显著（ p﹤0畅05）。随着Cu2＋浓度的增加，超氧化歧化酶（ SOD）活性、过氧化物酶（ POD）活性、可溶性蛋白质含量先升高后降低；过氧化氢酶（CAT）呈持续降低趋势，且与对照差异显著（p＜0畅05）。该研究结果为海菜花的保护提供了一定的理论支持。