岩生树花地衣型真菌NRPS基因的生物信息学分析

摘要

In order to searching the NRPS gene, the putative compound or protein of Ramalina intermedia lichen forming fungi, we obtained a novel NRPS gene(RiNRPS)by analysed the transcriptom of R.intermedia lichen forming fungi.We checked the RiNRPS gene using the BLAST, phylogenetic tree analysis and antiSMASH, NRPS predictor, NaPDoS, Norine bioinformatics, the results showed that: the relative molecular weight of the RiNRPS gene is 702 699.9, pI is 5.83, and unstable protein; the full length of RiNRPS open reading framer takes 19 098bp, and codes 6 362 amino acids, non-exist signal peptide, is an unsecreted protein, locates in the cytoplasmic matrix; it might code HC-toxin synthetase.The HC-toxin synthetase could catalyze Orn, Gln, Gln and Cys into cyclic tetrapeptides, the compound may be an polypeptides having the bioactivity of toxin, and the consti-tutional formula is C21H32N4O6.The study should apply some basis of gene mining of NRPS gene from Ramalina intermedia and the biosynthesis of nonribosomal peptides.%为了挖掘岩生树花地衣型真菌转录组数据中的NRPS 基因,找出其潜在化合物或蛋白,以岩生树花地衣型真菌的转录组数据为基础,分析获得一个新的NRPS基因(RiNRPS).利用BLAST比对、分子系统进化及an-tiSMASH、NRPS predictor、NaPDoS、Norine等生物信息学分析手段对岩生树花地衣型真菌RiNRPS基因进行分析,结果显示,RiNRPS基因相对分子质量为702699.9,等电点5.83,为不稳定蛋白;其开放阅读框总长19098 bp,编码6362个氨基酸;该蛋白不存在信号肽,为非分泌蛋白,定位于细胞质基质中;可能合成HC-toxin合成酶,可能的催化反应是以Orn(鸟氨酸)、Gln(谷氨酰胺)、Gln(谷氨酰胺)、Cys(半胱氨酸)4个氨基酸作为底物合成环状四肽,此环状四肽可能是一种具毒素活性的多肽、其结构式为C21H32N4O6.研究结果为以后岩生树花NRPS基因的挖掘及非核糖体多肽类物质的生物合成提供参考.