Production of fucoidanase from marine fungus Dendryphiella arenaroa TM94 by solid substrate fermentation using marine algae resources

摘要

A marine fungus strain TM94 isolated from sea sand was identified as Dendryphiella arenaria. It could produce fucoidanase by solid state fermentation using marine algae. The conditions for producing the enzyme were studied. The result showed that three marine substrates such as Laminaria sp. and Fucus vesiculosus and crude fucoidan could promote the fucoidanase production. Among three materials, crude fucoidan was the best substrate for Dendrypbiella arenaria TM94 to produce the enzyme. The highest fucoidanase activities reached 32.75 IU per gramme of medium after cultured 24 hours. The optimal temperature for fucoidanase yielding was 28℃. The best inoculum level for enzyme production by solid state culture was 2 ml spore suspension (1.0 x 10~7 spore/ml). The properties of enzyme were also studied. The result showed that the enzyme exhibited a maximal activity at 50℃ and at pH 4.6. The enzyme showed relatively stable below 40℃ and between pH 5.7 ～ 6.2. The enzyme could be applied in hydrolysis fucoidan.