DNA 条形码、形态学与地理分布相结合的植物果实（种子）鉴定方法--以口岸截获的外来入侵植物犬蔷薇（Rosa canina L．）为例

摘要

Species identification is difficult in the case of scarce references and highly trained professional.As a case study of an unknown fruit intercepted from vehicle entering from Xinjiang Port,we identified this unknown fruit by molecu-lar,morphological and geographical distribution methods.Based on ITS,atpF -H,psbK -I sequences,fruit morphology and geographical distribution,we finally identified it is Rosa canina L.which originated from Western Asia.The com-bined identification methods are simple and efficient,improving existing technology,and suitable for identifying plant fruits or seeds at the ports.%以单一方法鉴定物种具有参考文献（或比对数据）少、专业要求高的缺点。以新疆口岸进境货车截获的未知植物果实为例，从分子、形态、地理分布3个维度，对该未知果实逐级进行分类鉴定。根据 ITS、atpF －H、psbK －I 3段植物 DNA 通用条形码序列，以及形态学和地理分布信息，最终确定该未知果实为原产于西亚的蔷薇科蔷薇属犬蔷薇（Rosa canina L．）。本方法简单、准确，弥补了现有单一检测鉴定技术的不足，适用于进境产品中植物果实或种子的检疫鉴定。