果蔗'拔地拉'植株再生与农杆菌介导的遗传转化研究

摘要

Plant regeneration through callus induction were established by using young sheath of fruit sugarcane (Saccharum officenarum 'Badila'),and the transformation of calli mediated by Agrobacterium tumefaciens was studied.The optimum media were MS+2.0 mg L~(-1)2,4-D for calli induction,MS+2,4-D 2.0 mg L~(-1)+6-BA1 mg L~(-1)for differentiation;and MS+IAA2.0 mg L~(-1) for root induction,respectively.Plant expression vector pMG225,including crylA gene and CPTI gene,was transformed into calli of fruit sugarcane by Agrobacterium tumefaciens.rnIt confirmed that cryIAc gene had been integrated into fruit sugarcane genome by PCR and Southern blot analysis.%以果蔗(Sacchdrgm officenarum L.)'拔地拉'的幼嫩叶鞘为材料,以MS+2,4-D 2.0 mg L~(-1)为诱导培养基,MS+2,4-D 2.0 mg L~(-1)+6-BA 1.0 mg L~(-1)为分化培养基,MS+IAA2.0 mg L~(-1)为生根培养基,建立了高效的果蔗再生体系.利用农杆菌介导法将含有cryIA基因和CPTI基因的植物表达载体导入果蔗愈伤组织,经潮霉素筛选、PCR以及Southern杂交分析表明,cryIAc基因已整合进果蔗基因组中.