大叶山楝根化学成分与细胞毒活性的研究

摘要

In order to understand the antitumor active ingredients ofAphanamixis grandifoliaBl., nine compounds were isolated from 95% ethanol extract of its roots. They were identiifed as 7-hydroxycadalene (1), dregeana-1 (2), 4-oxopinoresinol (3), 4-ketopinoresinol (4), 6-deoxyjacareubin (5), schleicheol 1 (6), stigmasterol (7), β-sitosterol (8), and daucosterol (9) on the basis of spectral data. Compounds1–6 were isolated from the genusAphanamixis for the ifrst time, and the13C NMR spectral data of compound1 was reported for the ifrst time. Compounds1 and 5 showed inhibition of the proliferation of K562 cell line, and compounds1,5 and6 possessed inhibitory activity against SGC-7901 cell line.%为了解大叶山楝(Aphanamixis grandifoliaBl.)根中的抗肿瘤活性成分，利用各种色谱技术从其95%乙醇提取物中分离得到9个化合物，经波谱分析分别鉴定为：7-hydroxycadalene (1)、dregeana-1(2)、4-oxopinoresinol (3)、4-ketopinoresinol (4)、6-deoxyjacareubin (5)、schleicheol 1(6)、豆甾醇(7)、β-谷甾醇(8)和胡萝卜苷(9)。其中化合物1~6为首次从山楝属植物中分离得到，并首次报道了化合物1的碳谱数据。生物活性测试结果表明，化合物1和5对慢性髓原白血病细胞K562有生长抑制活性，化合物1、5和6对人胃癌细胞SGC-7901有生长抑制活性。