干扰素-γ、重组TGF-β1RⅡ 蛋白对小鼠日本血吸虫病肝纤维化的影响

摘要

目的 探讨IFN-γ、重组TGF-β1 RⅡ 蛋白对小鼠日本血吸虫病肝纤维化的影响.方法 昆明小鼠(6~8 周龄,体重18~25g,雌雄各半),随机分成6 组(6 只/组),正常对照组除外,其余30 只通过腹部皮肤感染日本血吸虫尾蚴18依2 条/鼠,六周后成功建立小鼠血吸虫性肝纤维化模型,具体治疗分组:淤正常对照组;于模型对照组;盂吡喹酮(PQT)治疗组;榆PQZ +IFN-γ治疗组;虞PQT+rTGF-β1RⅡ 蛋白治疗组;愚PQT+IFN-γ+ rTGF-β1 RⅡ 蛋白治疗组.第九周末处死小鼠,HE 染色法对肝组织进行病理学检查;TBA 法测肝匀浆MDA 含量.结果 IFN-γ、rTGF-β1RⅡ 蛋白治疗显著降低肝纤维化指数,抑制日本血吸虫诱导的肝MDA 水平的升高.结论 日本血吸虫病化学药物治疗中,以PQT 为基础配合rTGF-β1R域蛋白、IFN-γ的联合疗法策略优于PQT 单独用药策略;rTGF-β1R域蛋白、IFN-γ的抗纤维化效应可能与其抗氧化功能有关.%Objective To explore the effects of IFN-γ and rTGF-β1 RII on hepatic fibrosis in mice infected with schistosomiasis japonica. Methods 36 Kunming mice (6—8 w,body weight 18—22g, male and female in half) were randomly divided into 6 groups (n=6), 6 mice were nomal control group, 30 mice were challenged percutaneously with 18 ±2 cercariae of Schistosoma japonicum and six weeks later they were randomly divided into 5 groups: model control group, Praziquantel (PQT) group, PQT+IFN—γ group, PQT + rTGF- β1RII group, and PQT+IFN—γ+rTGF-β1RII group. All the mice were killed at the end of the 9th week. The area of egg granuloma and degree of hepatic fibrosis were observed via HE and Masson stainings. The concentrations of liver homogenates MDA were detected by spectrophotometer using Assay Kits. Results IFN—γ and /or TGF—β1RII protein significantly inhibited hepatic collagenous fibrosis in mice infected with schistosomiasis japonicum and reduced the concentrations of MDA that in liver tissue. Conclusion The combination of IFN—γ and sTGF—β1RII with PQT had better effect on hepatic fibrosis in mice infected with schistosomiasis japonica than the single use of PQT. The anti—fibrosis effect of IFN—γ and sTGF—β1RII might relate with its antiantioxidant function.