利用MT＇F法检测番木瓜种子中的异硫氰酸苄酯（BITC）在体外对肝癌细胞等6种常见癌细胞的生长抑制率。结果表明，番木瓜种子提取物中BITC的浓度达到5μm01·L-1（相当于0．745mg·L-1）时，对人肝癌HepG2细胞、人肺癌A549细胞、人乳腺癌MCF-7细胞、人大肠癌HCT-8细胞、人宫颈癌HeLa细胞、人前列腺癌DU-145细胞的生长抑制率均达到70％以上，其中，人肝癌细胞HepG2最敏感，C_BITC=2．5μmol·L。时抑制率可达87％，在C_BITC=10μmol·L-1和5．0μmol·L-1时，人大肠癌HCT-8细胞的生长抑制率几乎相同。因此，BITC具有良好的抑癌效果。%Benzyl isothiocyanate （BITC） was extracted from papaya seeds （Carica papaya L. ） and used to determine its inhibitory effect on the cell growth of 6 common cancers in vitro including liver cancer by using the MTr method. The results showed the papaya seed extract with BITC at the concentration of up to 5 μmol · L-1 （ equivalent to 0.745 mg · L-1 ） inhibited more than 70% of the cell growth of a human liver cancer cell line HepG2, a lung cancer cell line A549, a breast cancer cell line MCF-7, a colon cancer cell line HCT-8, a cervical cancer cell line Hela and a prostate cancer cell line DU-145. Of them the HepG2 liver cancer cells were the most sensitive to BITC which gave an 87% inhibition over the ceils at the concentration 2.5 μmol · L-1. HCT-8 colon cancer cells had a similar inhibition rate when treated with BITC at both 5.0 and 10μmol· L-1. This showed that higher concentration of BITC in the extract of papaya seeds was similar to the lower concentration （5.0 μmol · L-1 ） in inhibition of the growth of the cancer cells.
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