海洋真菌Aspergillus sp.SCS-KFD03的化学成分分析

摘要

A strain SCS-KFD03 of Aspergillus sp.was isolated from Sipunculus nudus collected from Haikou Bay,and fermented.Active constituents were isolated and purified from the fermentation broth and hyphae of the strain SCS-KFD03 by using various chromatographic techniques,including Silica gel,Sephadex LH-20 column and HPLC chromatography.Seven compounds (1-7) were extracted from the secondary metabolites of the strain SCS-KFD03 and identified as 3β,5 α-dihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (1),3β,4α-dihydroxy-26-methoxyer gosta-7,24 (28)-dien-6-one (2),(22E,24R)-ergosta-5,7,22-trien-3β-ol (3),leporin B (4),astellolide G (5),astellolide B (6) and astellolide A (7).The bio-activity evaluation of these compounds showed that compounds 2,3,5 and 6 had a certain antioxidant activity towards DPPH free radical.The compound 2 also exhibited a weak inhibitory activity against acetyl cholinesterase,and the compound 4 had a higher inhibitory activity against α-glucosidase than the positive control acarbose.%为了研究海洋真菌Aspergillus sp.SCS-KFD03次级代谢产物的活性成分,采用硅胶柱色谱、SephadexLH-20凝胶柱色谱和高效液相色谱等技术从其发酵液中分离得到7个化合物,运用各种波谱方法鉴定为3β,5 α-dihydroxy-(22E,24R)-ergosta-7,22-dien-6-one(1);3β,4α-dihydroxy-26-methoxyergosta-7,24(28)-dien-6-one(2);麦角甾醇(22E,24R)-ergosta-5,7,22-trien-3β-ol(3);leporinB(4);astellolide G(5);astellolide B(6)和astellolide A (7),并测定化合物的抗氧化活性、乙酰胆碱酯酶和α-糖苷酶抑制活性.结果表明:化合物2,3,5和6具有一定的抗氧化活性,化合物2具有较弱的乙酰胆碱酯酶抑制活性,化合物4具有强于阳性药阿卡波糖的α-糖苷酶抑制活性.