中性/碱性转化酶(Alkaline or neutral invertase,N/A-lnv)是木薯淀粉合成过程中的一种关键酶.笔者以木薯华南6号古铜期嫩叶为材料制备染色体标本,利用荧光原位杂交和原位PCR技术对N/A-lnv基因家族的11个成员进行了物理定位.结果表明,基因MeNINV5,MeNINV9和MeNINV10均位于第4号染色体上,其中基因MeNINV9和MeNINV10位于短臂上,到信号点的百分距离分别为68.52和95.35,基因MeNINV5位于长臂上,到着丝粒的百分距离为22.71;基因MeNINV4和nlNV1均位于第6号染色体长臂上,到着丝粒的百分距离分别为43.16和80.71;基因MeNINV6和MeNINV7分别位于第7号和第17号染色体的长臂上,信号点到着丝粒的百分距离分别是15.38和57.97;基因MeNINV1,MeNIN,V2,MeNINV3和MeNINV8分别位于第11号、第9号、第5号和第16号染色体的短臂上,信号位点到着丝粒的百分距离分别是40.10,51.88,91.75和76.33.中性/碱性转化酶基因家族内部部分成员之间存在连锁关系.研究结果可为木薯淀粉的高效积累机制及木薯种质遗传改良提供分子细胞遗传学依据.%Alkaline or neutral invertase (N/A-lnv) is a key enzyme for starch synthesis process in cassava.Chromosome specimens were prepared from the bronze-coloured tender leaves of cassava variety SC6,and 11 members of the cassava neutral or alkaline invertase gene family were physically localized by fluorescence in situ hybridization (FISH) and in situ PCR.The results showed that genes MeNINVS,MeNINV9 and MeNINV10 were located on the chromosome 4.MeNINV/9 and MeNINV10 had percentage distances of 68.52 and 95.35 from the signal locus to the centromere on the short arm,respectively,and MeNINV5 had a percentage distance of 22.71 from the signal locus to the centromere on the long arm.MeNINV4 and nINV1 genes were located on the long arm of chromosome 6,and their percentage distances from the signal locus to the centromere are 43.16 and 80.71,respectively;MeNINV6 and MeNINV7 genes were located on the long arms of chromosome 7 and 17,respectively,and their percentage distances from the signal locus to the centromere are 15.38 and 57.97,respectively;MeNINV1,MeNINV2,MeNINV3 and MeNINV8 genes were located on the short arms of chromosome 11,9,5 and 16,respectively,and their percentage distances from the signal locus to the centromere were 40.10,51.88,91.75 and 76.33,respectively.The linkage relationship was discussed between the N/A-lnv gene family and other genes located on cassava chromosomes.
展开▼
