睾酮水平的变化对雄性大鼠血管内皮功能的影响

摘要

Objective To investigate the effects of testosterone levels on vascular endothelial function in castrated male rats. Methods Thirty male Wistar rats were randomized into 3 groups: rats underwent sham operation (Sham group, n = 10), rats were castrated (Cas group, n = 10) and rats were castrated and supplemented with testosterone 2 mg/kg (TC group 2 d, n = 10). Blood concentrations of testosterone, nitric oxide (NO), endothelin-1 (ET-1) were measured after 8 weeks. Results Compared with the sham group, the plasma testosterone concentration in Cas group was significantly lower (P < 0.05), and that in Tc group was not significantly changed (P > 0.05); plasma NO level in Cas group was significantly lower(P <0.05), and no change in TC group(P > 0. 05); plasma ET-1 content in Cas was significantly higher(P <0. 05), and no significant change in TC group (P > 0.05). Compared with Cas group, plasma testosterone and NO concentrations were significantly increased; whereas plasma ET-1 level was reduced in TC group( P <0.05). Conclusion Change of testosterone levels can affect the vascular endothelial function in castrated male rats. Testosterone deficiency results in vascular endothelial damage, followed by reduced NO and increased ET-1.%目的 观察雄性大鼠去势后睾酮水平的变化对血管内皮功能的影响.方法 雄性Wistar大鼠30只随机化分为假手术组(10只)、单纯去势组(10只)和去势后+生理剂量睾酮组(补充睾酮组2d,10只；2 mg/kg).8周后测定3组大鼠血浆睾酮浓度,采用化学比色法测定血浆一氧化氮(NO)水平,ELISA法检测血清内皮素-1(ET-1)的含量.结果 与假手术组比较:单纯去势组血浆睾酮浓度明显降低(P＜0.05),补充睾酮组无明显变化(P＞0.05)；血浆NO水平单纯去势组显著降低(P＜0.05),补充睾酮组无明显变化(P＞0.05)；血浆ET-1含量单纯去势组明显高于假手术组(P＜0.05),而补充睾酮组无明显变化(P＞0.05).和单纯去势组相比:补充睾酮组血浆睾酮浓度显著增加,NO水平有明显升高,而血浆ET-1含量明显降低(均P＜0.05).结论 睾酮水平的变化可以影响去势术后大鼠血管内皮功能,睾酮缺乏可使大鼠血管内皮受损,释放NO减少以及ET-1增加.