Objective To study the biocompatibility of zinc-modified CaSiO3 bioceramic coating in vitro, and to provide experimental basis of its clinical application. Methods The physical characteristic of the coating and the concentration of differentions extracted from the coating were detected. Then the extract was used in MC3T3-E1 cells culture with different concentrations for cellular morphological observation, including the relative growth rate measured by MTT and the apoptosis rates detected by flow cytometry. Hemolysis ratio of the coating was also detected. Results Calcium, Silicon, Zinc ion concentrations of the coating's extract were significantly increased after immersion for 72 hours. All cells cultured with different proportions of diluent showed normal cellular morphology. Cells cultured in group 3.125% and 6.25% had larger quantity, higher relative increment rate and lower apoptosis rate, compared to positive control group (complete medium). Alone with the increasing concentrations, mild inhibition to the cells and increasing apoptosis rate were observed. The hemolysis rate of the coating's extract detected by hemolysis test was 0.5%. Conclusion The Zinc-modified CaSiO3 bioceramic coating with non-cytotoxicity and good biocompatibility has the biological basis of surface modification for bone implants and other biological material.%目的：研究锌修饰硅酸钙生物陶瓷涂层的体外生物相容性，为该涂层临床应用的可行性提供实验依据。方法检测该涂层的物理表征；检测该涂层浸提液的离子浓度；用不同稀释比例的浸提液培养MC3T3-E1细胞，观察细胞形态，MTT法检测细胞相对增殖率，并检测细胞凋亡水平；测定溶血率。结果该涂层浸泡72 h后，浸提液中钙、硅、锌离子浓度均明显升高；用不同稀释比例的浸提液培养的MC3T3-E1细胞都表现出正常的细胞形态，其中3.125%、6.25%组细胞量较多，相对增殖率高于阳性对照组，且细胞凋亡率低于阳性对照组（完全培养基）；随浸提液浓度增加，可对细胞增殖产生轻度抑制作用，凋亡率逐渐升高；溶血试验结果显示，该涂层浸提液的溶血率为0.5%。结论锌修饰硅酸钙生物陶瓷涂层没有细胞毒性、生物相容性好，具有作为骨植入体及其他生物材料表面改性的生物学基础。
展开▼
