β-内酰胺类抗生素对聚磷菌最小抑菌浓度的影响

摘要

The research detected the minimum inhibitory concentration (MIC) of β-lactam antibiotics to phosphorusaccumulating bacteria via trace broth dilution method.Amoxicillin,cefoperazone and aztreonam were selected as the experimental antibiotics,phosphorus-accumulating organisms (PAOs) as the test bacteria while Escherichia coli and Pseudomonas aeruginosa as the control strain.The result showed that the MIC of amoxicillin and cefoperazone to Escherichia coli were 4～8 μg/mL,0.25 ～0.5 μg/mL,while the MIC of aztreonam to pseudomonas aeruginosa was 2～4 μg/mL that conformed to CLSI standard.The MIC of amoxicillin,cefoperazoneandaztreonamto PAOs were 8 ～ 16 μg/mL,0.5 ～1 μg/mL and 4～8 μg/mL,respectively.%为本研究以聚磷菌作为受试生物,大肠埃希菌和铜绿假单胞菌作为质控菌属,采用微量肉汤稀释法,测定β-内酰胺类抗生素中阿莫西林、头孢哌酮、氨曲南对聚磷菌的最小抑菌浓度(MIC).结果显示,阿莫西林、头孢哌酮对大肠埃希菌的最小抑菌浓度分别为“4～8 μg/mL、0.25～0.5 μg/mL”,氨曲南对铜绿假单胞菌的最小抑菌浓度为“2～4μg/mL”,符合CLSI标准;阿莫西林、头孢哌酮、氨曲南对聚磷菌的最小抑菌浓度分别为“8～16 μg/mL、0.5～1 μg/mL、4～8 μg/mL”.