Objective: To compare the effects of six 3-sheet breaker (H102, H102-1, HI 02-2, H102-3, DH102, LD-7) on β-amyloid protein (Aβ) fibrous formation. Methods: The inhibitory effects of β-sheet breakers on Aβ1-42 fibrous formation were determined by using fluorescence analysis with Thioflavin T (ThT) and Fourier— transform infrared spectroscopy was used to study the secondary structure changes on aging Aβ in vitro. Results: 3-sheet breakers and vitamin E inhibited the Aβ-42 aggregation and formation of amyloid fibrosis with the percentage inhibitory as follows: (l4.168±0.038)%, (9.725±0.023)%, (22.535±0.013)%, (8.586±0.035)%, (5.37±0.019)%, (9.599±0.012)%, (12.736±0.026)%. FT-IR also revealed the best effect of H102-2 than others. Conclusion: H102-2 is much more efficient than the others 3-sheet breskers in inhibiting Aβ1-42 aggregation%目的:比较6种β片层阻断肽(H102、H102-1、H102-2、H102-3、DH102、LD-7)对β淀粉样蛋白(Aβ)聚集及纤维形成的影响,并进行药物筛选.方法:运用硫磺素T(ThT)荧光法以及傅里叶变换红外光谱法(FT-IR)分析6种β片层阻断肽对Aβ1-42聚集的影响.结果:6种β片层阻断肽及对照组维生素E对Aβ1-42聚集和纤维抑制率分别为:( 14.168±0.038)％、(9.725±0.023)％、(22.535±0.013)％、(8.586±0.035)％、(5.37±0.019)％、(9.599±0.012)％、( 12.736±0.026)％,提示H102-2的效果最佳;FT-IR结果也以H102-2的效果最佳.结论:H102-2对β淀粉样蛋白聚集和纤维抑制效果最佳.
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