PRRX1的表达对卵巢癌血管生成拟态形成及患者预后的影响

摘要

Objective:To investigate the paired related homeobox 1 (PRRX1) and its pathway molecules expression in ovarian cancer and its relationship with the vasculogenic mimicry (VM) formation,so as to explore PRRX1 effect on metastasis and prognosis in ovarian cancer.Methods:The expressions of PRRX1,Wnt5a,β-catenin as well as VM in ovarian cancer samples from 62 cases were examined by immunohistochemical and C D31/periodic acid-Schiff double staining.And the relationships between PRRX 1 and VM formation,Wnt5a,β-catenin were assessed by x2 and pearson tests.The prognostic values of PRRX1 were measured by Kaplan-Meier survival analysis.Results:PRRX1 expression was associated with FIGO stage,metastasis and VM formation (P ＜0.05).No significant correlations were found between PRRX1 and age,tumor size,histological type,degree of differentiation of the tumor (P ＞0.05).PRRX1 overexpression was found to have the relationship with β-catenin nuclear expression but showed no relationship with Wnt5a and β-catenin cytoplasm expression.Both PRRX1 expression and VM formation were correlated with ovarian cancer metastasis and prognosis.Kaplan-Meier survival analysis showed that patients with PRRX1 expression and VM formation had shorter survival than PRRX1-or VM-patients,respectively.And poor prognosis was observed.PRRX1 expression and β-catenin nuclear expression had shorter survival than PRRX1-or β-catenin nuclear negative expression (P ＜0.05),where this relationship showed no significance between PRRX1 and Wnt5a,β-catenin cytoplasm expression (P ＞0.05).Conclusion:PRRX1 could regulate VM formation in ovarian cancer through the activation of Wnt/β-catenin signaling,indicating poor prognosis,so PRRX 1 might be a candidate marker for the diagnosis and prognosis predictor of ovarian cancer.%目的:检测配对相关同源框1 (PRRX1)及其通路分子在卵巢癌中表达情况以及其与血管生成拟态(VM)的关系,推测PRRX1对卵巢癌患者预后的影响.方法:采用免疫组化法检测62例卵巢癌中PRRX1、Wnt5a、β-catenin的表达情况,采用CD31/PAS双染法检测卵巢癌中VM的情况.采用x2检验和Pearson相关分析来分析PRRX1与VM形成和临床病理资料之间的关系以及PRRX1与Wnt5a、B-catenin的关系.采用Kaplan-Meier法评估PRRX1对卵巢癌患者生存预后的影响.结果:PRRX1表达对卵巢癌患者的FIGO分期、转移及VM形成有影响且具有统计学意义(P＜0.05),而对患者的年龄、肿瘤大小、组织学类型、肿瘤分化程度的影响无明显统计学差异(P＞0.05).PRRX1表达与β-catenin的核表达有关,与Wnt5a、β-catenin的浆表达无关.PRRX1阳性表达和有VM形成的患者生存时间比PRRX1阴性表达或无VM形成的患者短(P＜0.05)且预后差;PRRX1阳性表达和β-catenin核阳性表达的患者生存时间短于PRRX1阴性表达或β-catenin核阴性表达的患者(P＜0.05);而PRRX1阳性表达与Wnt5a阳性表达、β-catenin的浆阳性表达的患者生存时间与PRRX1阴性表达或Wnt5a阴性表达、β-catenin的浆阴性表达的患者生存时间相比,差别无意义(P＞0.05).结论:PRRX1通过Wnt/β-catenin信号通路调节VM形成从而影响卵巢癌患者预后,PRRX1可作为卵巢癌诊断和预后预测的指标.