EGCG-O-甲基转移酶(EOMT)在重组大肠杆菌中的表达条件研究

摘要

EGCG3"Me could be produced from EGCG catalyzed by EGCG-O-Methyltransferase. Taken the yield of EGCG3"Me as main index, the present study focused on the producing conditions of EGCG-O-Methyltransferase induced by IPTG in recombinant E. Coli bacteria. Results showed that the optimum producing conditions were as follows: the concentration of IPTG was 0.05 mmol/L, the induction time was 20 h, the initial pH of medium was 7.0 and the induction temperature was 20℃.%本研究以EGCG-O-甲基转移酶(EOMT)催化EGCG生成EGCG3”Me的产量为主要指标,探讨了重组大肠杆菌内EGCG-O-甲基转移酶的诱导表达条件.结果表明,当诱导剂IPTG终浓度为0.05 mmol/L,诱导时间为20h,培养基初始pH为7.0,以及诱导温度为20℃时,EOMT的表达效果最佳.